间歇供氧策略在光合细菌污水生物转化系统中的应用  

Application of periodic oxygen supplementation strategy in photosynthetic bacteria wastewater bio-conversion system

作　　者：单钰 何仕超 卢海凤[1,2,3] 张光明 蒋伟忠 王朝元[1,2] 李保明[1,2] SHAN Yu;HE Shichao;LU Haifeng;ZHANG Guangming;JIANG Weizhong;WANG Chaoyuan;LI Baoming(College of Water Resources and Civil Engineering,China Agricultural University,Beijing 100083;Sanya Institute of China Agricultural University,Sanya 572000;Key Laboratory of Agricultural Engineering in Structure and Environment of MOARA,Beijing 100083;College of Energy and Environmental Engineering,Hebei University of Technology,Tianjin 300131)

机构地区：[1]中国农业大学水利与土木工程学院,北京100083 [2]中国农业大学三亚研究院,三亚572000 [3]农业农村部设施农业工程重点实验室,北京100083 [4]河北工业大学能源与环境工程学院,天津300131

出　　处：《环境科学学报》2025年第3期135-144,共10页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划(No.2022YFE0135600);海南省外国专家计划(No.G20230607005E)。

摘　　要：光合细菌(Photosynthetic bacteria,PSB)污水资源化技术可实现污水中营养物质的高值生物质转化,具有环保与经济的双重效益.溶解氧(Dissolved oxygen,DO)是PSB生长的重要环境因子,其合理控制对系统的稳定运行及资源化效率至关重要.本研究考察了间歇供氧策略在PSB污水资源化技术中应用的可行性,并探究了其对PSB污水资源化中试规模下运行性能的影响.结果表明,间歇供氧策略用于PSB污水资源化系统的潜力较大.间歇供氧策略下,PSB生物量最高可达2186.80 mg·L^(-1),比连续厌氧组和连续供氧组分别高81.34%与27.19%.厌氧-供氧间歇策略下COD去除率(90.87%~91.12%)与连续供氧组相当,但显著高于连续厌氧条件(65.68%).同时,间歇供氧组的NH_(4)^(+)-N去除率(70.50%~80.90%)介于连续供氧组(91.96%)与连续厌氧(65.20%)组之间.通过污水与菌液的混合循环流动可实现中试规模管式光生物反应器内间歇供氧的稳定运行.系统COD和NH_(4)^(+)-N去除率分别为80.12%~98.55%和61.25%~84.03%,PSB生物量可达1272.80~2617.85 mg·L^(-1).此外,中试系统的单位面积生物量、蛋白质、类胡萝卜素和菌绿素日产量分别为36.1~84.2 g·m^(-2)·d^(-1)、15.04~33.90 g·m^(-2)·d^(-1)、27.06~77.22 mg·m^(-2)·d^(-1)和189.71~444.91 mg·m^(-2)·d^(-1),证明了PSB污水资源化系统以间歇供氧模式半连续运行具有实际应用的可行性.The photosynthetic bacteria(PSB)wastewater resource utilization technology can realize high-value biomass conversion of nutrients in wastewater,with both environmental protection and economic benefits.Dissolved oxygen(DO)is a critical environmental factor for the growth of PSB,and its proper control is crucial for the stable operation and resource utilization efficiency of the system utilization.This study investigated the feasibility of periodic oxygen supplementation(A-O)strategy in the PSB wastewater resource utilization technology and explored the influence on the operational performance of the system at a pilot scale.The results indicated that A-O strategy can be used in PSB wastewater resource utilization system with high potential.Under A-O strategy,the highest PSB biomass can reach 2186.80 mg·L^(-1),which was 81.34%and 27.19%higher than the continuous anaerobic(N-O)and continuous aerobic(C-O)strategies,respectively.The COD removal of A-O(90.87%~91.12%)was comparable to C-O(92.18%),but significantly higher than N-O(65.68%).The NH_(4)^(+)-N removal of A-O(70.50%~80.90%)was between C-O(91.96%)and N-O(65.20%).Stable operation of A-O within a pilot-scale tubular photobioreactor can be realized through the mixed circulation flow of wastewater and bacterial solution.The system COD removal and NH_(4)^(+)-N removal were 80.1%~98.5%,61.2%~84.0%,and PSB biomass concentration was 1272.8~2617.8 mg·L^(-1).In addition,the unit area yield of biomass,protein,carotenoids,and BChl were 36.1~84.2 g·m^(-2)·d^(-1),15.0~33.9 g·m^(-2)·d^(-1),27.1~77.2 mg·m^(-2)·d^(-1),and 189.7~444.9 mg·m^(-2)·d^(-1),which demonstrated the feasibility of the PSB wastewater resource utilization system operating semicontinuously in A-O strategy for practical applications.

关 键 词：光合细菌 溶解氧 间歇供氧 管式光生物反应器 中试系统 

分 类 号：X703[环境科学与工程—环境工程]