正交试验法优选消渴健脾方有效成分丹酚酸B提取工艺  

Optimization of the Extraction Process of Salvianolic Acid B,An Active Ingredient of Xiaoke Jianpi Presciption by Orthogonal Test Method

作　　者：朱晓东 刘艺 张佳佳 ZHU Xiaodong;LIU Yi;ZHANG Jiajia(Urumqi Hospital of Traditional Chinese Medicine,Urumqi 830002,China)

机构地区：[1]乌鲁木齐市中医医院,新疆乌鲁木齐830002

出　　处：《中国民族民间医药》2025年第5期58-61,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：2023年乌鲁木齐市中医药骨干人才培养项目(乌中医函{2024}1号-19);2022年自治区青年岐黄学者项目(新卫科教函{2021}26号)。

摘　　要：目的:优选消渴健脾方中有效成分丹酚酸B的提取工艺。方法:以甲醇-5%乙酸(35∶65),柱温23℃,流速为1.0 mL/min,检测波长为281 nm,建立丹酚酸B的HPLC色谱条件;采用正交试验设计对加水量、煎煮时间、煎煮次数3个因素进行考察,优选消渴健脾方中有效成分丹酚酸B的提取工艺。结果:优选的最佳提取条件为加水量10倍、煎煮时间45 min、煎煮次数2次。验证试验结果表明,在此方法下消渴健脾方中有效成分丹酚酸B的含量平均值为0.882 mg/g。结论:本研究优选的提取工艺稳定、可靠,可用于消渴健脾方有效成分丹酚酸B的提取。Objective To optimize the extraction process of salvianolic acid B,an active ingredient in Xiaoke Jianpi Formula.Methods The HPLC chromatographic conditions of salvianolic acid B were established using methanol-5%acetic acid(35∶65),column temperature of 23℃,flow rate of 1.0 mL/min,and detection wavelength of 281 nm.Three factors,including the amount of added water,the decoction time,and the number of times of decoction were investigated using orthogonal experimental design,to optimize the extraction process of salvianolic acid B.Results The optimal extraction process was 10 times of water addition,45 min of decoction time and 2 times of decoction.The results of the validation test showed that the average content of salvianolic acid B in the formula was 0.882 mg/g.Conclusion The preferred extraction process is stable and feasible,and can be used for the extraction of salvianolic acid B.

关 键 词：消渴健脾方 正交试验 丹酚酸B 提取工艺 

分 类 号：R284.2[医药卫生—中药学]