LC-MS法测定水田七中根薯酮内酯A和根薯酮内酯B  

Analysis of Taccalonolide A and Taccalonolide B in Schizocapsa Plantaginea Rhizoma by Liquid Chromatography-Mass Spectrometry

作　　者：覃丽郦 何颂华 罗轶 谷立勍 QIN Lili;HE Songhua;LUO Yi;GU Liqing(Guangxi Institute for Drug Control—NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine,Chinese Material Medica,Nanning 530021,China)

机构地区：[1]广西药品检验研究院/国家药品监督管理局中药材质量监测与评价重点实验室,广西南宁530021

出　　处：《中国民族民间医药》2025年第5期65-67,110,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西药品监督管理局科研计划(桂药监科2019-10);广西常用壮瑶药药材质量标准研究(桂科AB17292069)。

摘　　要：目的:建立水田七中根薯酮内酯A和根薯酮内酯B的液质联用检测(LC-MS)和分析方法。方法:采用Kromasil Proshell C_(18)柱(2.5μm,2.1 mm×100 mm)色谱柱,以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,多反应监测模式进行检测分析。结果:根薯酮内酯A和根薯酮内酯B进样量分别在10.1136~505.6800 pg和0.9996~99.9600 pg范围内线性关系良好,r>0.999,加样回收率分别为95.45%(RSD 2.88%),99.12%(RSD 2.82%)。结论:所建立的方法快速、准确、灵敏、可靠,能够用于水田七中根薯酮内酯A和根薯酮内酯B的测定,为其质量控制研究提供科学依据。Objective To establish the analysis method of taccalonolide A and taccalonolide B in Schizocapsa Plantaginea Rhizoma by liquid chromatography-mass spectrometry(LC-MS).Methods An Kromasil Proshell C_(18)(2.5μm,2.1 mm×100 mm)column was used with gradient elution of acetonitrile-0.1%formic acid.The multiple-reaction monitoring mode was used.As a result,the LC-MS method was established to detect the taccalonolide A and taccalonolideB in Schizocapsa Plantaginea Rhizoma.And the method was further validated and used to test all samples.Results The developed method showed good linearity in the range of 10.1136-505.6800 pg,0.9996-99.9600 pg with r>0.999 for taccalonolide A and taccalonolide B,respectively.The recovery rates were 95.45%(RSD 2.88%),99.12%(RSD 2.82%),respectively.Conclusion The established method is accurate,sensitive and reliable.It can be applied for analysis of taccalonolide A and taccalonolide B in Schizocapsa Plantaginea Rhizoma.And it can provide sound scientific basis for the study of Schizocapsa plantaginea rhizome quality control.

关 键 词：水田七 根薯酮内酯A 根薯酮内酯B 液质联用 

分 类 号：R917[医药卫生—药物分析学]