UFLC-QTOF-MS/MS分析噁唑酰草胺及其在生物体内的代谢产物  

Analysis of matamifop and its metabolic products in biological systems via UFLC-QTOF-MS/MS

作　　者：罗剑文[1] 钟创光[1] 刘羿飞 黄欣如 黄昆 彭霏 殷梓宁 何昀泽 张以顺[1] LUO Jianwen;ZHONG Chuangguang;LIU Yifei;HUANG Xinru;HUANG Kun;PENG Fei;YIN Zining;HE Yunze;ZHANG Yishun(School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报(自然科学版)(中英文)》2025年第2期67-75,共9页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省高等教育学会实验室管理专业委员会基金(GDJ20220159);中山大学校级教学质量与教学改革工程类项目(33000-12220011)。

摘　　要：采用超高效液相色谱-四极杆串联飞行时间质谱(UFLC-QTOF-MS/MS)技术,以黄粉虫幼虫(Tenebrio molitor)作为模式生物,探索了一种在缺乏全面代谢物标准品的情况下发现和积累噁唑酰草胺(metamifop)代谢物色谱-质谱特征数据的新方法,并构建出噁唑酰草胺在黄粉虫中的代谢途径。研究显示从连续15 d定量摄食含噁唑酰草胺2 mg/g药饵的黄粉虫粪便提取物中发现了21种代谢产物,包括14种Ⅰ相代谢产物和7种Ⅱ相代谢产物。在Ⅰ相代谢产物中除单氧化噁唑酰草胺、精噁唑禾草酸、6-CBO、HPFMPA、HPPA和HFMPA已报道外,其余8种均为首次在生物体内发现。并发现这些I相代谢产物至少通过与氨基酸结合、与葡萄糖硫酸酯结合以及与硫酸酯结合3种途径进一步转化为相应的Ⅱ相代谢产物。Utilizing ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UFLC-QTOF-MS/MS)technology,and employing the larvae of mealworm(Tenebrio molitor)as a model organism,this study developed a new approach to discover and accumulate chromatographic-mass spectrometric characteristic data of metamifop metabolites in the absence of comprehensive metabolite standards.The metabolic pathway of metamifop in T.molitor was further elucidated.The study identified 21 metabolites in the excreta of mealworms larvae that were fed a diet containing 2 mg/g of metamifop over a period of 15 days.These included 14 phase I metabolites and 7 phaseⅡmetabolites.Among the phase I metabolites,beside the reported compounds such as Metamifop monooxide,Fenoxaprop acid,6-CBO,HPFMPA,HPPA,and HFMPA,eight were identified for the first time in any biological system. It was also observed that these phase I metabolites underwenttransformation into corresponding phase Ⅱ metabolites through three primary pathways: Conjugationwith amino acids, glucose-sulfate esterification, and sulfation.

关 键 词：噁唑酰草胺(metamifop) 黄粉虫(Tenebrio molitor) 代谢产物 超高效液相色谱-四极杆串联飞行时间质谱(UFLC-QTOF-MS/MS) 

分 类 号：Q-331[生物学] S482.46[农业科学—农药学]