Exploring the material basis and mechanism of Moringa oleifera in alleviating slow transit constipation based on network pharmacology and animal models  

作　　者：Sijin Li Xiaoyu Gao Kaifeng Guo Shuangfeng Liu Weiqian Yang Yan Zhao Jun Sheng Zhongbin Bai Yang Tian 

机构地区：[1]Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650500,China [2]College of Food Science and Technology,Yunnan Agricultural University,Kunming 650500,China [3]Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China [4]Department of Science and Technology,Yunnan Agricultural University,Kunming 650201,China [5]College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China

出　　处：《Food Science and Human Wellness》2025年第3期902-913,共12页食品科学与人类健康(英文)

摘　　要：Moringa oleifera have laxative effects,but their active compositions and mechanisms are not very clear thus far.To this end,we systematically explored the active components and mechanism of M.oleifera leaves in relieving constipation by using the slow transit constipation(STC)mouse model and network pharmacology.The results of animal experiments showed that M.oleifera aqueous extract(MOA)had good laxative activity,and its 70%alcohol soluble part(ASP)also showed significant laxative activity(P<0.01).Network pharmacological prediction results suggested that L-phenylalanine(Phe)was the key compound of ASP,and it might relieve constipation through tachykinin receptor 1(TACR1)and three kinds of adrenergic receptors,includingα_(1A)(ADRA1A),α_(2A)(ADRA2A),andα_(2B)(ADRA2B).Further animal experiment results showed that Phe significantly promoted gastrointestinal motility.Phe may relieve STC by enhancing the release of substance P(SP)and upregulating the m RNA expression of TACR1 in the ileum.Importantly,Phe may also promote intestinal movement by downregulating the m RNA expression of ADRA2A and ADRA2B and upregulating the m RNA expression of Calm and the m RNA and protein expression of myosin light chain 9 in the ileum,thereby activating the G protein-coupled receptor-myosin light chain signaling pathway.These results lay a foundation for the application of M.oleifera and Phe in constipation.

关 键 词：Moringa oleifera Network pharmacology L-PHENYLALANINE Gastrointestinal motility LAXATIVE 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]