Novel silver nanoparticles loaded Cu-based metal-organic framework as a promising antimicrobial material for controlling Escherichia coli O157:H7 in pork  

作　　者：Dongbo Yang Jing Yi Xiaoya Li Shuqi Zhang Yiming Xiang Xingxing Liu Zhongguo Shan Haihua Wang 

机构地区：[1]College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China

出　　处：《Food Science and Human Wellness》2025年第3期914-924,共11页食品科学与人类健康(英文)

基　　金：funded by the Key Research and Development Projects of Shaanxi Province(2023-YBNY-178);Shaanxi Fundamental Science Research Project for Chemistry&Biology(22JHQ029);China Postdoctoral Science Foundation(2019M653766);National Natural Science Foundation of China(21901212)。

摘　　要：The risk of infection following consumption of foodborne pathogens contaminated foods became a significant concern for human health and imposes great economic losses to food industry.Herein,Ag nanoparticles were integrated to Cu-based metal-organic framework(Cu-MOF)for antibacterial activity.The crystal structure,morphology and composition of the prepared composite Ag@Cu-MOF were confirmed by powder X-ray diffraction,thermogravimetric analysis,scanning electron microscope,transmission electron microscope,Fourier transform infrared spectroscopy,and X-ray photoelectron spectroscopy.Antibacterial assays revealed that Ag@Cu-MOF exhibited increased inhibitory activity against Escherichia coli O157:H7 in comparison to Cu-MOF.Ag@Cu-MOF treated bacterial cells displayed distinct morphological changes,a decreased ratio of live/dead cells,as well as a reduction of intracellular ATP.Antibiofilm studies demonstrated that Ag@CuMOF could dramatically inhibit biofilm formation and disrupt preformed biofilms by interfering the metabolic activity and decreasing the expression of biofilm-associated genes.Food contamination model illustrated that Ag@Cu-MOF significantly prevented the growth of E.coli O157:H7 in packed pork.This study sheds light on the potential of Ag@Cu-MOF as a promising antimicrobial material for preserving pork.

关 键 词：Metal-organic frameworks ANTIMICROBIAL ANTIBIOFILM Pork model 

分 类 号：TB3[一般工业技术—材料科学与工程]