Comparative metabolomics reveal histamine biodegradation mechanism by salt stressed Bacillus subtilis JZXJ-7  

作　　者：Rundong Wang Yijia Deng Yuhao Zhang Xuepeng Li Ravi Gooneratne Jianrong Li 

机构地区：[1]College of Food Science and Engineering,Lingnan Normal University,Zhanjiang 524048,China [2]College of Food Science,Southwest University,Chongqing 400715,China [3]College of Food Science and Engineering,Bohai University,Jinzhou 121013,China [4]Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing,Chongqing 400715,China [5]Department of Wine,Food and Molecular Biosciences,Faculty of Agriculture and Life Sciences,Lincoln University,Christchurch 7647,New Zealand

出　　处：《Food Science and Human Wellness》2025年第3期925-940,共16页食品科学与人类健康(英文)

基　　金：supported by the National Key R&D Program of China(2019YFD0901702);National Natural Science Foundation of China(32201929,32202134);2025 Guangdong Basic and Applied Basic Research Foundation of Natural Science Foundation Project(2025A1515011748);High-level Talent Project of Lingnan Normal University(ZL2415);China Postdoctoral Science Foundation(2022M712625);2021 and 2022 Chongqing Postdoctoral Special Funding Project。

摘　　要：Bacillus subtilis JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear.This study aims to evaluate the effect of 170 and 340 mmol/L Na Cl on B.subtilis JZXJ-7 growth,histamine degradation,antioxidant enzymes(catalase(CAT),superoxide dismutase(SOD)and glutathione S-transferase(GST))activities and Na^(+)/K^(+)-ATPase activity.Furthermore,comparative metabolomics was used to investigate histamine biodegradation mechanism by B.subtilis JZXJ-7 subjected to salt stress.Both 170 and 340 mmol/L Na Cl promoted B.subtilis JZXJ-7 growth in late stages of reproduction(32-48 h),increased h istamine degradation rate by 64.85%and 79.87%(P<0.05),Na^(+)/K^(+)-ATPase activity to 6.28(P<0.05)and 11.63 U/mg(P<0.01)respectively.N a Cl treatment significantly increased the activities of CAT,GST and SOD(P<0.05),a mino acids and its metabolites(33.39%),benzene and substituted derivatives(12.05%),heterocyclic compounds(10.62%),organic acids and derivatives(9.75%),aldehydes,ketones,esters(5.59%)and nucleotides and its metabolites(4.58%).Metabolite set enrichment analysis revealed Na Cl induced differential metabolic pathways of D-glutamine,D-glutamate,L-arginine,L-proline,histidine and glycerophospholipids,L-lysine degradation,and aminoacyl-t RNA biosynthesis.Exposure to 340 mmol/L Na Cl up-regulated carbohydrate,glutathione and glycerophospholipid metabolism.The new insights into the mechanism of salt stress to promote B.subtilis JZJX-7 growth,energy metabolic pathways and to degrade histamine provide the theoretical basis for application of B.subtilis JZXJ-7 in food fermentation industry.

关 键 词：Bacillus subtilis JZXJ-7 HISTAMINE Salt stress Antioxidant enzymes Metabolomics 

分 类 号：O62[理学—有机化学]