Desiccation and osmotic resistance mechanism of Cronobacter spp.isolated from powdered infant formula  

作　　者：Shiqian Fu Danliangmin Song Xue Qin Lihan Wang Qianyu Zhao Chaoxin Man Xinyan Yang Yujun Jiang 

机构地区：[1]Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province,Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition,College of Food Science and Engineering,Ningbo University,Ningbo 315800,China [2]Key Laboratory of Dairy Science,Ministry of Education,Department of Food Science,Northeast Agricultural University,Harbin 150030,China

出　　处：《Food Science and Human Wellness》2025年第3期968-980,共13页食品科学与人类健康(英文)

基　　金：supported by the Joint Funds of the National Natural Science Foundation of China(U21A20272)。

摘　　要：Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.

关 键 词：Powdered infant formula Cronobacter spp. Desiccation resistance Osmotic resistance TRANSCRIPTOMICS 

分 类 号：R56[医药卫生—呼吸系统]