An innovative indirect competitive immunosorbent approach for precise detetion aflatoxin B_(1) based on high-affinity immunoglobulin G coupled CuO-anchored Fe_(3)O_(4)nanozymes  

作　　者：Xueqin Wang Chuan Liu Haizhen Tao Xuanping Zhao Mengna Wan Junyan Liu Na Li 

机构地区：[1]College of Bioengineering,Henan University of Technology,Zhengzhou 450001,China [2]Henan Institute of Medical and Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001,China [3]Henan Provincial Key Laboratory of Ultrasound Imaging and Artifi cial Intelligence,Henan Provincial People’s Hospital,Zhengzhou University People’s Hospital,Zhengzhou University,Zhengzhou 450001,China

出　　处：《Food Science and Human Wellness》2025年第3期1017-1025,共9页食品科学与人类健康(英文)

基　　金：supported by the Scientific and Technological Project of Henan Province(232102321117);National Natural Science Foundation of China(82202198);the National Engineering Research Center of Wheat and Corn Further Processing of Henan University of Technology(NL2022010);Project of Basic Research Fund of Henan Provincial Institute of Medical and Pharmacological Sciences(2023BP0106);the Innovative Funds Plan of Henan University of Technology(2020ZKCJ23)。

摘　　要：Aflatoxin B1(AFB1)is a carcinogenic toxin naturally produced in most food crops that severely threaten human health,and effective methods are urgent to improve the detection accuracy.Herein an indirect competitive immunosorbent approach was elaborately developed based on high-affinity immunoglobulin G(IgG)coupled CuO-anchored Fe_(3)O_(4)nanozymes for precise and ultrasensitive detection of AFB_(1)in food crops including peanut,corn and wheat.The high-affinity nanozymes were fabricated by the assembly of inner core Fe_(3)O_(4)nanoparticles and mesoporous silica capping layer,Cu O further situated within large aperture of the coating layer via in-situ growth,and then conjugated with ligand rabbit anti-mouse Ig G,which can specifically bind with AFB_(1).The results showed the hybrid high-affinity nanozymes displayed enhanced peroxidasemimic activities and catalytic performances,achieving a linear range of 0.06-61.93(lg(ng/mL))and a detection limit of 0.0037 ng/mL,30 times better than that of the conventional enzyme-linked immunosorbent assay.The constructed nanozymes were successfully applied to the detection of AFB_(1)in food products with an average spiked recovery of 96.53%and relative standard deviations less than 2.8%.Therefore,the accurate hybrid nanozymes may serve for AFB_(1)detection in various foods in future.

关 键 词：Aflatoxin B_(1) Enhanced peroxidase-mimic activities Nanozyme based enzyme-linked immunosorbent assay Food crops 

分 类 号：O64[理学—物理化学]