SMAD4对多囊卵巢综合征大鼠卵巢颗粒细胞增殖和凋亡的调控作用  

SMAD4 regulates the proliferation and apoptosis of ovarian granulosa cells in rats with polycystic ovary syndrome

作　　者：董安琪 郑金丹 于晓萌 刘丽丽 DONG Anqi;ZHENG Jindan;YU Xiaomeng;LIU Lili(Departments of Obstetrics and Gynecology,The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China;Departments of Obstetrics and Gynecology,Women and Children’s Hospital of Jinzhou,Jinzhou 121001,China)

机构地区：[1]锦州医科大学附属第一医院妇产科,辽宁锦州121001 [2]锦州市妇婴医院妇产科,辽宁锦州121001

出　　处：《中国医科大学学报》2025年第3期223-227,共5页Journal of China Medical University

基　　金：辽宁省科学技术计划(2022-MS-394)。

摘　　要：目的探讨SMAD4对多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞增殖和凋亡的影响。方法脱氢表雄酮(DHEA)法构建PCOS大鼠模型,体外提取并培养颗粒细胞。实时定量PCR、Western blotting检测大鼠卵巢颗粒细胞中SMAD4表达水平。SMAD4-siRNA转染PCOS大鼠卵巢颗粒细胞,实时定量PCR检测转染后细胞中SMAD4 mRNA水平,Western blotting检测转染后细胞中SMAD4、PCNA、BAX、BCL-2蛋白表达水平。CCK-8法检测siRNA干扰PCOS大鼠卵巢颗粒细胞后细胞生长情况。结果HE染色显示,PCOS组大鼠卵巢卵泡空泡增多,颗粒细胞层数和黄体数明显减少,造模成功。FSHR阳性率大于95%。与对照组相比,PCOS大鼠卵巢颗粒细胞中SMAD4表达水平升高(P<0.05)。siRNA可显著降低PCOS大鼠卵巢颗粒细胞中SMAD4表达水平(P<0.01),并可促进颗粒细胞增殖、抑制凋亡。结论PCOS大鼠卵巢颗粒细胞发育障碍可能与SMAD4基因过表达有关,SMAD4有望成为治疗PCOS患者排卵障碍的潜在靶点。Objective To examine the effects of SMAD4 on the proliferation and apoptosis of ovarian granulosa cells in rats with polycystic ovary syndrome(PCOS).Methods A PCOS rat model was established using DHEA,and ovarian granulosa cells were extracted and cultured in vitro.The expression of SMAD4 in ovarian granulosa cells was detected by quantitative real-time PCR and Western blotting.SMAD4-siRNA was transfected into ovarian granulosa cells from PCOS rats.The expression of SMAD4 mRNA after transfection was determined by quantitative real-time PCR.Western blotting was performed to detect the expression levels of PCNA,BAX,and BCL-2 proteins after transfection.A CCK-8 assay was performed to evaluate cell growth after siRNA interference.Results The HE staining results revealed that the number of ovarian follicular vacuoles increased and that the number of granulosa cell layers and corpus luteum decreased,thus indicating the establishment of a PCOS model.The FSHR positivity rate exceeded 95%.SMAD4 expression in ovarian granulosa cells was higher in the PCOS group than in the control group(P<0.05).Furthermore,siRNA effectively reduced SMAD4 expression in ovarian granulosa cells of PCOS rats(P<0.01),promoted proliferation,and inhibited the apoptosis of granulosa cells.Conclusion The hindered growth of ovarian granulosa cells in PCOS rats may be linked to the overexpression of SMAD4 mRNA,which suggests that targeting SMAD4 could be a promising approach for treating ovulatory abnormalities in patients with PCOS.

关 键 词：多囊卵巢综合征 颗粒细胞 SMAD4 

分 类 号：R711.7[医药卫生—妇产科学]