脂肪干细胞源性外泌体对成纤维细胞及烧伤创面愈合的影响  

Effect of adipose-derived stem cell exosome on fibroblasts and wound healing

作　　者：左娜 陶凯 唐琪 于猛 ZUO Na;TAO Kai;TANG Qi;YU Meng(Department of Burn and Plastic Microsurgery,The Fourth Affiliated Hospital of China Medical University,Shenyang 110012,China)

机构地区：[1]中国医科大学附属第四医院烧伤整形显微外科,沈阳110012

出　　处：《中国医科大学学报》2025年第3期233-237,共5页Journal of China Medical University

基　　金：辽宁省科学技术计划(2021JH2/10300108)。

摘　　要：目的探讨脂肪干细胞源性外泌体(ADSC-Exo)对成纤维细胞增殖、迁移能力以及大鼠深Ⅱ度烧伤创面愈合的影响。方法培养人成纤维细胞,采用ADSC-Exos或PBS处理细胞,通过CCK-8和划痕实验观察ADSC-Exo对成纤维细胞增殖能力和迁移能力的影响。选取40只SD大鼠构建深Ⅱ度烧伤模型,分为Exo组和PBS组,Exo组于烧伤创面周边注射ADSC-Exo溶液,PBS组注射等量PBS溶液。观察2组大鼠创面愈合情况,通过HE染色观察炎症细胞浸润情况,通过免疫组织化学CD31染色观察创面血管新生情况。结果CCK-8实验结果显示,ADSC-Exo可促进成纤维细胞增殖,且呈浓度依赖性(P<0.05);划痕实验结果显示,ADSC-Exo可增强成纤维细胞的迁移能力(P<0.0001)。动物实验发现,创面周边注射ADSC-Exo可促进烧伤创面愈合(P<0.0001)。与PBS组比较,Exo组大鼠烧伤创面炎症细胞浸润减少,CD31表达增加(P<0.01)。结论ADSC-Exo可促进成纤维细胞增殖、迁移,并能减轻创面炎症、促进新生血管生成,从而促进烧伤创面愈合。Objective To investigate the effect of adipose-derived stem cell exosome(ADSC-Exo)on the proliferation and migration of fibroblasts as well as on wound healing of deep second degree burns in rats.Methods Fibroblasts were cultured with ADSC-Exo or in phosphate-buffered saline(PBS).The effect of ADSC-Exo on the proliferation and migration of fibroblasts was observed using the CCK-8 assay and scratch test.Forty Sprague-Dawley(SD)rats were selected to construct a deep second-degree burn model and divided into the exosome and control groups.The exosome group was injected with the ADSC-Exo solution around the wound,and the control group was injected with an equal amount of PBS solution.Wound healing was observed in both groups.Inflammatory infiltration was observed using hematoxylin and eosin(HE)staining,and wound angiogenesis was observed using CD31 immunohistochemical staining.Results The CCK-8 assay revealed that ADSC-Exo promoted fibroblast proliferation in a concentration-dependent manner.The results of the scratch test showed that ADSC-Exo significantly enhanced the migratory ability of fibroblasts(P<0.0001).The wound healing results showed that ADSC-Exo injection promoted burn wound healing in rats(P<0.0001).Pathological analysis indicated that inflammatory cell infiltration was less and CD31 expression was higher in the ADSC-Exo group than in the control group(P<0.01).Conclusion ADSC-Exo promoted the proliferation and migration of fibroblasts,reduced wound inflammation,promoted neovascularization,and enhanced wound healing.

关 键 词：脂肪干细胞源性外泌体 成纤维细胞 创面愈合 

分 类 号：R644[医药卫生—外科学]