Gnetum montanum extract ameliorates ethanol-induced hepatic injury and metabolic dysfunction via inhibition of xanthine oxidase  

作　　者：Hong-Linh Tran Thuy-Duong Nguyen Thu-Hang Nguyen Hai-Nam Nguyen Duc-Vinh Pham 

机构地区：[1]Department of Pharmacology,Hanoi University of Pharmacy,Hanoi,Vietnam [2]Department of Medicinal Chemistry,Hanoi University of Pharmacy,Hanoi,Vietnam

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2025年第3期98-108,共11页亚太热带生物医学杂志(英文版)

基　　金：funded by Vietnam National Foundation for Science and Technology Development under grant number 108.05-2023.23.

摘　　要：Objective:To investigate the effects of a crude extract from Gnetum montanum Markgr.on ethanol-induced hepatotoxicity and metabolic disorders.Methods:Alcoholic liver disorder was induced in mice by administering increasing doses of ethanol via oral gavage.Biomarkers of liver injury and oxidative stress were assessed at the end of the study.Liver tissue damage and fat deposition were evaluated using hematoxylin and eosin and oil red O staining,respectively.In addition,key biomarkers were examined in acetaldehyde-treated HepG2 cells.Results:Ethanol consumption induced characteristic pathological changes,including elevated serum markers of liver injury,hepatic lipid accumulation,and oxidative stress in liver tissues.Oral administration of Gnetum montanum extract(175 and 350 mg/kg)decreased serum aspartate aminotransferase,alanine aminotransferase,γ-glutamyl transferase,and bilirubin levels in ethanol-treated mice.The extract also lowered triglyceride levels in serum and liver tissue in a dose-dependent manner.Furthermore,it mitigated malondialdehyde levels,preserved reduced glutathione levels,and enhanced catalase activity and total antioxidant capacity in liver tissue homogenates.Additionally,ethanol-induced hyperuricemia was suppressed by Gnetum montanum extract by inhibiting xanthine oxidase activity.Similar effects were observed in Gnetum montanum extract-treated HepG2 cells.Conclusions:This study demonstrates that Gnetum montanum extract alleviates ethanol-induced hepatic injury by alleviating oxidative stress and inhibiting xanthine oxidase activity.

关 键 词：Alcoholic liver disease Gnetum montanum Hepatic steatosis Hepatoprotective effect Xanthine oxidase 

分 类 号：X70[环境科学与工程—环境工程]