草珊瑚挥发油对越南槐圆斑病病原菌的抑制作用  

The inhibitory effects of volatile oil of Sarcandra glabra on the Didymella glomerata of Sophora tonkinensis

作　　者：丘卓秋 蒋妮[1,2] 詹鑫婕 宋利沙 李林轩[1] 黄琦 QIU Zhuo-qiu;JIANG Ni;ZHAN Xin-jie;SONG Li-sha;LI Lin-xuan;HUANG Qi(Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs/Guangxi Chinese Medicinal Materials Breeding Engineering Technology Research Center;National Center for Traditional Chinese Medicine Inheritance and Innovation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园,广西道地药材高品质形成与应用重点实验室/广西中药材良种繁育工程技术研究中心,南宁530023 [2]广西壮族自治区药用植物园,国家中医药传承创新中心,南宁530023

出　　处：《湖北农业科学》2025年第2期89-93,244,共6页Hubei Agricultural Sciences

基　　金：肉桂等广西特色药材高质量生产关键技术及系列产品开发研究项目(桂科AA22096021);广西中医药多学科交叉创新团队项目(GZKJ2305);广西道地药材高品质形成与应用重点实验室项目(ZJC2020003)。

摘　　要：探明草珊瑚[Sarcandra glabra(Thunb.)Nakai]挥发油对越南槐(Sophora tonkinensis Gagnep.)圆斑病病原菌(Didymella glomerata)的抑菌活性及作用机理。通过测定菌落直径及菌丝体质量,研究挥发油对越南槐圆斑病病原菌生长的抑制作用;通过测定电导率、可溶性蛋白质含量、还原糖含量、丙二醛(MDA)含量,初步探讨挥发油的作用机制。结果表明,草珊瑚挥发油对越南槐圆斑病病原菌具有显著抑菌活性,EC_(50)为271.00 mg/L;挥发油质量浓度2 000 mg/L、作用72 h对菌丝生长的抑制效果最好,菌落扩展和菌丝生长抑制率分别为100%、95.00%;处理的电导率、可溶性蛋白质含量、MDA含量均显著高于对照,挥发油质量浓度2 000 mg/L、处理72 h的电导率和可溶性蛋白质含量分别是对照的1.54倍和4.74倍;MDA含量在处理96 h达到最大值,是对照的3.11倍;处理还原糖含量变化不大,对照还原糖含量随时间的延长而降低,比处理72 h降低了52.37%。The antibacterial activity and mechanism of the volatile oil of Sarcandra glabra(Thunb.)Nakai against Didymella glomera⁃ta of Sophora tonkinensis Gagnep.were investigated.The inhibitory effects of Sarcandra glabra oil on the growth of Didymella glomera⁃ta were studied by measuring the colony diameter and mycelium weight.The electrical conductivity,soluble protein,reducing sugar and malondialdehyde(MDA)were determined to investigate the action mechanism of Sarcandra glabra oil.The results showed that the volatile oil of Sarcandra glabra had significant antibacterial activity against the Didymella glomerata of Sophora tonkinensis,and the EC_(50) was 271.00 mg/L.The inhibitory effect on mycelial growth was the best when the concentration of volatile oil was 2000 mg/L and the treatment time was 72 h.The inhibitory rate of colony expansion and mycelial growth were 100%and 95.00%,respectively.The electrical conductivity,soluble protein content and MDA content of the treatment group were significantly higher than those of the con⁃trol group.The electrical conductivity and soluble protein content of the treatment were 1.54 times and 4.74 times of those the control,respectively when the mass concentration of volatile oil was 2000 mg/L for 72 h.MDA reached its maximum value at 96 h,which was 3.11 times that of the control.The reducing sugar content in the treatment did not change much,and the reducing sugar content in the control group decreased with the extension of time,and was 53.36%lower than that in the treatment group at 72 h.

关 键 词：草珊瑚[Sarcandra glabra(Thunb.)Nakai] 越南槐(Sophora tonkinensis Gagnep.) 挥发油 圆斑病病原菌(Didymella glomerata) 抑制作用 

分 类 号：S432.44[农业科学—植物病理学] R284.1[农业科学—农业昆虫与害虫防治]