绞股蓝多糖对热应激小鼠卵巢氧化损伤的保护作用  

Protection of Gynostemma pentaphyllum polysaccharide on oxidative damage of ovary in mice under heat stress

作　　者：陈蕾晓 何欢 欧阳露 牟容凤 赖鹏羽 王晓丽[1] CHEN Lei-xiao;HE Huan;OUYANG Lu;MOU Rong-feng;LAI Peng-yu;WANG Xiao-li(College of Animal Science and Technology,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学动物科学技术学院,南宁530004

出　　处：《湖北农业科学》2025年第2期144-149,共6页Hubei Agricultural Sciences

基　　金：广西大学“大学生创新创业训练计划”项目(S202110593148)。

摘　　要：为探究不同剂量绞股蓝多糖(Gynostemma pentaphyllum polysaccharides,GPP)对热应激小鼠卵巢氧化损伤的保护作用,采用酶联免疫吸附、免疫组化等研究方法,分别对空白对照组(Control)、热应激对照组(HS)、高剂量绞股蓝多糖组(GPP-H)、中剂量绞股蓝多糖组(GPP-M)、低剂量绞股蓝多糖组(GPP-L)中小鼠的体重和卵巢指数,卵巢中各级卵泡数量、最大直径及卵巢皮质厚度,卵巢中各类生殖激素和雌激素β型受体(Estrogen receptor β,ERβ)的表达水平,血清中抗氧化能力以及卵巢颗粒细胞凋亡率测定分析。结果表明,与HS组相比,GPP各剂量组小鼠体重显著升高(P<0.05);GPP-H组原始卵泡数显著升高(P<0.05),GPP-L、GPP-H组闭锁卵泡数显著减少(P<0.05),GPP各剂量组卵巢皮质厚度、原始卵泡、次级卵泡和黄体最大直径显著升高(P<0.05);GPP各剂量组GnRH、E_(2)和P_(4)含量均显著升高(P<0.05),GPP-H组FSH含量显著升高(P<0.05);GPP各剂量组GSH-Px酶活力显著升高(P<0.05),GPP-M组TSOD酶活力显著升高(P<0.05),GPP各剂量组MDA含量显著降低(P<0.05)。GPP可能通过发挥ER激动剂的类固醇样作用,改善垂体和卵巢内分泌功能,恢复、保护卵巢储备能力,增强酶类抗氧化系统的活力,缓解由热应激造成卵巢的氧化损伤,GPP-H组剂量效果较佳。In order to explore the protective effect of different doses of Gynostemmapentaphyllum polysaccharides(GPP)on oxidative damage of ovary in mice caused by heat stress,enzyme-linked immunosorbent assay(ELISA),immunohistochemical techniques and other research methods were used to observe and analyze the body weight and ovarian index,the number of follicles at all levels,the maximum diameter and the thickness of ovarian cortex,various reproductive hormones in ovary,the level of estrogen receptorβex⁃pression,antioxidant capacity in blood serum and the apoptosis rate of ovarian granulosa cells.The results showed that compared with group HS,the weight of mice in different doses of GPP was significantly higher(P<0.05);the number of primordial follicles in group GPP-H was significantly higher(P<0.05);the number of atretic follicles in groups GPP-L and GPP-H was significantly lower(P<0.05);the thickness of ovarian cortex,the maximum diameters of primordial follicle,primary follicle and corpus luteum in different doses of GPP were significantly larger(P<0.05);the secretion of GnRH,E_(2) and P_(4) in different doses of GPP was significantly higher,the secretion of FSH in group GPP-H was significantly higher(P<0.05);GSH-Px activity indifferent doses of GPP was significantly higher(P<0.05),T-SOD activity in group GPP-M was significantly higher(P<0.05),the content of MDA in different doses of GPP was significantly lower(P<0.05).In conclusion,GPP could improve the endocrine function of pituitary and ovary,restore and protect the ovarian reserve capacity,enhance the activity of enzyme antioxidant system,and alleviate the oxidative damage of ovary caused by heat stress by exerting the steroid-like effect of ER agonist,in which GPP-H worked most efficiently.

关 键 词：卵巢 氧化损伤 热应激 绞股蓝多糖 小鼠 

分 类 号：S852[农业科学—基础兽医学]