大叶黄精和黄精中与有机酸代谢相关的bHLH转录因子家族分析  

Analysis of bHLH transcription factors family related to organic acid metabolism in Polygonatum kingianum var.grandifolium and Polygonatum sibiricum Red

作　　者：朱天润 万小林 粱思琪 肖强 ZHU Tian-run;WAN Xiao-lin;LIANG Si-qi;XIAO Qiang(School of Forestry and Horticulture/Hubei Key Laboratory of Biologic Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,Hubei,China)

机构地区：[1]湖北民族大学林学园艺学院/生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《湖北农业科学》2025年第2期202-206,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31260057);湖北省科技厅技术创新重大专项(2019ACA120)。

摘　　要：采用测序平台对大叶黄精(Polygonatum kingianum var. grandifolium)和黄精(Polygonatum sibiricum Red)转录组进行测序,利用生物信息学方法对与有机酸代谢相关的bHLH转录因子家族进行鉴定,对预测的蛋白质开展理化性质、结构域以及系统进化关系的分析。结果表明,基于大叶黄精和黄精的转录组数据,共获得转录因子46 682个,其中bHLH转录因子110个;总转录数据中具有显著差异的转录因子共978个,其中bHLH转录因子共53个;结合代谢组预测数据,得到与有机酸相关的转录因子31个,其中与有机酸相关的bHLH转录因子22个。除Cluster-43865.11外,Motif 1在其他序列中均有分布,绝大多数序列均含Motif 4;22个bHLH转录因子家族成员均存在不同程度的保守基序变异现象,每个bHLH含有1~14个Motif,平均含有7.81个Motif。The transcriptomes of Polygonatum kingianum var.grandifolium and Polygonatum sibiricum Red were sequenced using a sequencing platform.Bioinformatics methods were used to identify the bHLH transcription factors family related to organic acid metabo⁃lism,and the predicted proteins were analyzed for their physicochemical properties,structural domains,and phylogenetic relation⁃ships.The results showed that based on the transcriptome data of Polygonatum kingianum var.grandifolium and Polygonatum sibiri⁃cum Red,a total of 46682 transcription factors were obtained,including 110 bHLH transcription factors;there were 978 transcription factors with significant differences in the total transcription data,including 53 bHLH transcription factors;based on metabolomic pre⁃diction data,31 transcription factors related to organic acid were identified,including 22 bHLH transcription factors related to organic acid.Except for Cluster-43865.11,Motif 1 was distributed in all other sequences,and the vast majority of sequences contained Motif 4;all 22 members of the bHLH transcription factors family exhibited varying degrees of conserved motif variation,with each bHLH containing 1-14 Motifs and an average of 7.81 Motifs.

关 键 词：大叶黄精(Polygonatum kingianum var.grandifolium) 黄精(Polygonatum sibiricum Red) 有机酸 bHLH转录因子 生物信息学 家族 鉴定 

分 类 号：S567.239[农业科学—中草药栽培] R284.1[农业科学—作物学]