吲哚-3-乙酸抑制剂PPBa对Cd胁迫下水稻幼根糖代谢和激素含量的影响  

Effects of indole-3-acetic acid inhibitor PPBa on sugar metabolism and hormone content in rice seedling roots under cadmium stress

作　　者：涂子豪 杨思蓥 曾研华[1] 郭琳 曾勇军[1] TU Zihao;YANG Siying;ZENGYanhua;GUO Lin;ZENGYongjun(School ofAgronomy Sciences,JiangxiAgricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学农学院,江西南昌330045

出　　处：《生物灾害科学》2025年第1期1-10,共10页Biological Disaster Science

基　　金：国家重点研发计划(2023YFD2301304)。

摘　　要：【目的】镉(Cd)是水稻生产中常见的重金属污染物,其过量吸收会对水稻的激素和糖类代谢的交叉互作产生影响。PPBa(4–苯醚基苯硼酸)作为一种高效的生长素(IAA)抑制剂,通过抑制IAA合成,与植物的逆境胁迫响应关系密切。研究旨在探究不同浓度的Cd胁迫处理下,PPBa(4–苯醚基苯硼酸)对水稻幼根中糖-激素以及矿质营养元素含量的影响。【方法】以中嘉早17为试验材料,设置4个水平的Cd浓度处理,分别是0,10,40,70μmol/L;2个水平的PPBa处理,分别为含0.1%(v/v)二甲基亚砜(DMSO)的蒸馏水溶液,含50μmol/L PPBa+0.1%(v/v)DMSO的蒸馏水溶液,系统分析PPBa对Cd胁迫下水稻幼根生理生化特性的影响。【结果】(1)PPBa加剧了Cd胁迫下糖类物质的代谢失衡,导致幼根渗透压失衡。重度Cd胁迫下加入PPBa显著降低了根中可溶性糖和蔗糖含量,降幅分别为44.8%和34.9%;(2)PPBa显著抑制了重度Cd胁迫下植物激素分泌,其中吲哚乙酸(IAA)、脱落酸(ABA)和茉莉酸(JA)的含量分别下降了42.7%、46.1%和41.8%。【结论】研究揭示了PPBa在Cd胁迫下对水稻幼根糖-激素代谢及矿质营养元素含量的调控机制,为深入理解植物对重金属胁迫的耐受性及内源调控机制提供了理论依据。[Objective]Cadmium(Cd)is a common heavy metal pollutant in the production of rice in Southern China with acid red soil.Excessive Cd absorption by rice affects the phytohormones and sugar contents,and thus disturbs the rice growth.As an efficient inhibitor of IAA,PPBa(4-phenylbenzeneboronic acid)is closely related to the plant’s response to heavy metal stress.This study aims to explore how different concentrations of Cd stress induce changes in phytohormones,and thereby cross-talk with sugar and elements in rice seedling roots.[Method]In the study,Zhongjiazao 17 was cultivated in four levels of Cd concentration treatment,namely 0μmol/L,10μmol/L,40μmol/L,and 70μmol/L,combining with two levels of PPBa treatment,distilled water containing 0.1%(v/v)dimethyl sulfoxide(DMSO),and distilled water containing 50μmol/L PPBa+0.1%(v/v)DMSO.[Result](1)The YUCCA enzyme inhibitor PPBa caused an imbalance in sugar content,exacerbating root osmolytes imbalance.The addition of PPBa significantly reduced the soluble sugar and sucrose contents in the roots,with decreases of 44.8%and 34.9%.(2)The addition of PPBa inhibited hormone secretion in plants under severe Cd stress,with indole-acetic acid decreasing by 42.7%,abscisic acid decreasing by 46.1%,and jasmonic acid decreasing by 41.8%.[Conclusion]This study elucidates the regulatory mechanism of PPBa on sugar-hormone metabolism and elemental contents in rice roots under Cadmium(Cd)stress,providing a theoretical foundation for a deeper understanding of plant tolerance to heavy metal stress and endogenous regulatory mechanisms.

关 键 词：IAA抑制剂 CD胁迫 根系 糖 激素 矿质营养元素 

分 类 号：S511[农业科学—作物学]