胶体金技术在hnRNP A1蛋白检测中的应用前景  

The application prospect of colloidal gold technology in the detection of hnRNP A1 protein

作　　者：刘佳蓉 黄晶 张宇欢 孔德丞 胡琼 孔令保[2] LIU Jiarong;HUANG Jing;ZHANG Yuhuan;KONG Decheng;HU Qiong;KONG Lingbao(College of Biological Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China;Key Laboratory of Animal Viruses and Genetic Engineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]江西农业大学动物病毒与基因工程重点实验室,江西南昌330045

出　　处：《生物灾害科学》2025年第1期134-140,共7页Biological Disaster Science

基　　金：国家自然科学基金项目(32360908);江西省自然科学基金重点项目(20224ACB205004);南昌市动物病毒与基因工程重点实验室(2021-NCZDSYS-008)。

摘　　要：hnRNP A1是一种参与转录调控和m RNA剪接等细胞调控的RNA结合蛋白,在发现之初被鉴定为端粒DNA结合蛋白,但随着研究的深入,发现其与神经退行性疾病及癌症等多种疾病相关。高效准确的hnRNP A1检测对于疾病诊断和治疗至关重要。随着生物医学技术的飞速发展,胶体金免疫层析技术因其快速、简便和低成本的优势,在蛋白质分析中广泛应用。通过探讨胶体金色免疫层析技术用于检测hnRNP A1的可能性及其在操作、速度和成本上的优势,为早期诊断和治疗提供新思路。hnRNP A1 is an RNA-binding protein involved in cellular regulation,including transcriptional regulation and mRNA splicing.The protein was initially identified as a telomeric DNA-binding protein and further found to participate in various diseases,such as neurodegenerative disorders and cancer.Therefore,efficient and accurate detection of hnRNP A1 is critical for hnRNP A-associated disease diagnosis and therapy.With the rapid advancement of biomedical technology,colloidal gold immunochromatography has been widely used in the protein analysis due to its advantages of rapidity,simpleness,and low-cost.This article explores the possibility of using this technique for the detection of hnRNP A1 and highlights its advantages in operation,speed,and cost,providing new insights for early diagnosis and treatment.

关 键 词：hnRNP A1蛋白 胶体金技术 免疫层析 疾病诊断:试剂条开发 

分 类 号：R446.5[医药卫生—诊断学]