慢性心理应激模型小鼠中医证型变化规律研究  

Study on patterns of traditional Chinese medicine syndrome changes in mouse models of chronic psychological stress

作　　者：薛茜尹 吴淑雅 许婧余 张怡[1] 张铭珈 敖海清[1] XUE Qianyin;WU Shuya;XU Jingyu;ZHANG Yi;ZHANG Mingjia;AO Haiqing(School of Public Health and Management,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;School of Basic Medicine,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]广州中医药大学公共卫生与管理学院,广州510006 [2]浙江中医药大学基础医学院,杭州310053

出　　处：《中国实验动物学报》2025年第2期180-189,共10页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81673838);国家自然基金青年项目(82205229);广东省区域联合基金-青年基金项目(2021A1515110798)。

摘　　要：目的 研究慢性心理应激模型小鼠在造模过程中的证型变化规律及其可能的物质基础。方法采用慢性温和不可预知应激(chronic unpredictable mild stress, CUMS)方法制备慢性心理应激小鼠模型,造模2周后在造模同时使用逍遥散(XYS组)和四逆散(SNS组)治疗,比较造模小鼠(M4组和M6组)和给药2周(S4组和X4组)、4周(S6组和X6组)后慢性心理应激小鼠的一般情况、行为学和生化指标。结果 与M4组小鼠比较,S4组和X4组小鼠体质量、旷场实验总路程和中央路程、血清去甲肾上腺素(norepinephrine, NE)浓度、胃部Ghrelin表达均升高;S4组小鼠糖水偏好率、血清D-木糖浓度均升高;与X4组比较,S4组小鼠旷场实验总路程、血清D-木糖浓度均升高;与M6组小鼠比较,S6组和X6组小鼠体质量、血清NE浓度、血清D-木糖浓度、胃部Ghrelin表达均升高;X6组小鼠糖水偏好率、旷场实验总路程、穿梭箱实验主动逃避次数均升高,血清皮质酮(cortisol, CORT)浓度降低;与S6组相比,X6组小鼠体质量、旷场实验总路程、血清D-木糖浓度、胃部Ghrelin表达均升高。结论 慢性心理应激小鼠造模过程中中医证型由肝郁气滞证演变为肝郁脾虚证,其证候变化生物学基础可能是血清CORT和NE浓度组合变化及胃中Ghrelin水平下降。Objective To investigate the patterns of syndrome changes and their possible material basis in mouse models of chronic psychological stress during the modeling process.Methods A chronic unpredictable mild stress(CUMS)method was employed to prepare a model of chronic psychological stress in mice.After 2 weeks of modeling,Xiaoyao powder(XYS group)and Sini powder(SNS group)were administered concurrently with the modeling process.General conditions,behavioral,and biochemical indicators were compared between the modeling mice(M4 and M6 group)and the mice treated for 2 weeks(S4 and X4 group)and 4 weeks(S6 and X6 group)after chronic psychological stress.Results Compared with M4 group mice,body mass,total distance and central distance in the open field test,serum NE concentration,and gastric Ghrelin expression were increased in S4 group and X4 group mice,and sugar water preference rate and serum D⁃xylose concentration were increased in S4 group mice.Compared with X4 group mice,total distance in the open field test and serum D⁃xylose concentration were increased in the S4 group mice.Compared with M6 group mice,body mass,serum NE concentration,serum D⁃xylose concentration,and gastric Ghrelin expression were increased in group S6 and X6 group mice;sugar water preference rate,total distance in the open field test,and active avoidance counts in the shuttle box test were increased in X6 group mice,while their serum CORT concentration was decreased.Compared with S6 group mice,body mass,total distance in the open field test,serum D⁃xylose concentration,and gastric Ghrelin expression in X6 group mice were increased.Conclusions During the process of modeling chronic psychological stress in mice,the traditional Chinese medicine syndrome evolved from liver qi stagnation to liver qi stagnation with spleen deficiency.The biological bases of the syndrome changes may be the combined changes in serum CORT and NE concentrations and the decrease in gastric Ghrelin levels.

关 键 词：慢性心理应激 情志致病 中医证型 辨证论治 

分 类 号：Q95-33[生物学—动物学]