2,4-二硝基氯苯诱导BALB/c荷瘤小鼠特应性皮炎模型的建立  

Establishment of an atopic dermatitis model in tumor⁃bearing BALB/c mice induced by 2,4⁃dinitrochlorobenzene

作　　者：牛晓涛 左佳倩 黄玉洁 任雯沁 束昕妍 舒鹏[1,2] NIU Xiaotao;ZUO Jiaqian;HUANG Yujie;REN Wenqin;SHU Xinyan;SHU Peng(the Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,南京210029 [2]南京中医药大学,南京210029

出　　处：《中国实验动物学报》2025年第2期216-224,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：江苏省中医药科技发展计划重点项目(ZD202214);国家自然科学基金(82374539);江苏省研究生实践创新计划(KYCX24_2230)。

摘　　要：目的 本研究使用2,4-二硝基氯苯(2,4-dinitrochlorobenzene, DNCB)诱导BALB/c荷瘤小鼠建立特应性皮炎(atopic dermatitis, AD)模型,模拟临床恶性肿瘤患者皮肤炎症并发症的情况。方法 将BALB/c小鼠分为不同组别:空白对照组(NC group),模型组(MODEL group),特应性皮炎组(AD group),地塞米松组(DEX group)。将模型组和地塞米松组的小鼠进行S-180肿瘤细胞腋下接种后,利用DNCB刺激模型组、特应性皮炎组、地塞米松组小鼠的背部皮肤和耳部,建立荷瘤小鼠特应性皮炎动物模型。观察和记录小鼠体质量的变化,在末次给药后评估小鼠背部皮肤状况,取脾计算脾系数,测定小鼠耳片质量差以计算耳廓肿胀度和肿胀抑制率,并对背部皮肤组织进行组织病理学检测,使用ELISA法检测血清中IgE、TNF-α、IL-4及IL-17水平。结果 与空白对照组相比,模型组、特应性皮炎组、地塞米松组小鼠的皮肤出现了红斑、丘疹、鳞屑和苔藓样改变,同时伴有体质量下降,脾系数和耳廓肿胀度明显增加。病理结果表明,皮肤结构不完整,皮肤厚度显著增加,有大量炎性细胞浸润,肥大细胞数量增加。血清中IgE、TNF-α、IL-4和IL-17水平均有所上升。与模型组相比,地塞米松组在各项检测指标上均有改善。结论 DNCB激发可成功建立荷瘤小鼠特应性皮炎动物模型,该模型具有药物可控性,这为进行恶性肿瘤与皮肤炎症的相关科学研究提供了有益的科研工具。Objective In this study,2,4⁃dinitrochlorobenzene(DNCB)was used to induce the establishment of an atopic dermatitis(AD)model in BALB/c homozygous mice to simulate the skin inflammatory complications in patients with clinical malignancies.Methods BALB/c mice were divided into different groups:negative control group(NC group),model group(MODEL group),atopic dermatitis group(AD group),and dexamethasone group(DEX group).After the mice in MODEL group and DEX group were inoculated with S⁃180 tumor cells in the axilla,MODEL group,AD group and DEX group were stimulated with DNCB on the dorsal skin and the ear to establish an animal model of atopic dermatitis in tumor⁃bearing mice.Changes in body weight were observed and recorded,the dorsal skin condition of mice was assessed after the last administration of the drug,the spleen was taken to calculate the spleen coefficient,the difference in the mass of mouse ear slices was determined to calculate the degree of auricular swelling and the rate of inhibition of swelling,and histopathological tests were performed on the dorsal skin tissues to detect the levels of IgE,TNF⁃α,IL⁃4,and IL⁃17 in the serum using an ELISA assay.Results Compared with the NC group,the skin of mice in the MODEL and AD groups showed erythematous,papular,scaly and mossy changes,accompanied by weight loss,and a significant increase in splenic coefficient and auricular swelling.Pathologic findings showed an incomplete skin structure,a significant increase in skin thickness,a large infiltration of inflammatory cells,and an increase in the number of mast cells.Serum levels of IgE,TNF⁃α,IL⁃4 and IL⁃17 were increased.Compared with the MODEL group,the DEX group showed an improvement in all the assays.Conclusions DNCB excitation can successfully establish an animal model of AD in hormonal mice,which is drug⁃controllable,which provides a useful scientific tool for conducting scientific research related to malignant tumors and skin inflammation.

关 键 词：动物模型 特应性皮炎 恶性肿瘤 BALB/C小鼠 

分 类 号：Q95-33[生物学—动物学]