电针对糖尿病神经痛大鼠背根神经节TRPV4表达的影响  

Effect of electroacupuncture on the expression of TRPV4 in the dorsal root ganglion of diabetic neuropathic pain model rats

作　　者：江民健 池恒愉 康玉蓉 蒋永亮[1,2,3] 郑崯沐 李斯苡 周姝婷 刘伯宇 邵晓梅 方剑乔[1,2,3] 何晓芬[1,2,3] JIANG Minjian;CHI Hengyu;KANG Yurong;JIANG Yongliang;ZHENG Yinmu;LI Siyi;ZHOU Shuting;LIU Boyu;SHAO Xiaomei;FANG Jianqiao;HE Xiaofen(Third Clinical Medical College,Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China;Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053,China;Institute of Acupuncture and Moxibustion,Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053 [2]浙江省针灸神经病学研究重点实验室,杭州310053 [3]浙江中医药大学针灸研究院,杭州310053

出　　处：《中国实验动物学报》2025年第2期241-248,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省自然科学基金项目(ZCLY24H2701,LY22H270006);国家中医药管理局科技司-浙江省共建项目重点项目(GZY-ZJ-KJ-24073);浙江中医药大学附属医院专项(2022FSYYZZ09,2024FSYYZY05)。

摘　　要：目的 观察电针(electroacupuncture, EA)对糖尿病神经痛(diabetic neuropathic pain, DNP)大鼠体质量、空腹血糖、热痛阈、背根神经节(dorsal root ganglion, DRG)瞬时受体电位香草蛋白4(transient receptor potential vanilloid 4,TRPV4)的影响。方法 采用向腹腔注入链脲佐菌素(streptozotocin, STZ)构建DNP大鼠模型。第15~21天,在大鼠双侧足三里(ST36)和昆仑(BL60)穴予以每日一次30 min的2 Hz EA治疗。检测各组大鼠体质量、空腹血糖和热痛阈。采用免疫荧光检测大鼠L4~L6 DRG中TRPV4和神经元(neuron, NeuN)共标的表达。检测TRPV4激动剂GSK1016790A对EA干预DNP大鼠体质量、空腹血糖、热痛阈的影响。结果 第7天后,与正常组相比,模型组体质量明显下降(P<0.01),空腹血糖明显上升(P<0.01);第21天,与模型组相比,模型+电针组热痛阈明显提高(P<0.01);大鼠L4~L6 DRG上的TRPV4和NeuN共标免疫荧光结果显示:模型组的阳性细胞表达较正常组均显著升高(P<0.01),模型+电针组大鼠L4~L6 DRG TRPV4和NeuN共标的阳性细胞表达较模型组均显著下降(P<0.01);TRPV4激动剂GSK1016790A可翻转EA对DNP大鼠热痛阈下调作用(P<0.01)。结论 EA能减轻链脲佐菌素诱导的DNP,其作用机制可能与其抑制DRG中TRPV4的表达有关。Objective To explore the function of electroacupuncture(EA)on body mass,fasting blood glucose,heat pain threshold,and transient receptor potential vanilloid 4(TRPV4)in the dorsal root ganglia(DRG)of rats with diabetic neuropathic pain(DNP).Methods A DNP rat model was formed by intraperitoneally injecting the animals with STZ.From days 15 to 21,bilateral Zusanli and Kunlun points of the DNP rat model were treated with electroacupuncture once daily for 30 min.We then measured their body mass,fasting blood glucose,and heat pain threshold.The co⁃expression of TRPV4 and NeuN in the rat L4~L6 DRG was detected by immunofluorescence.The effects of the TRPV4 agonist GSK1016790A on body mass,fasting blood glucose,and the heat pain threshold of DNP rats treated with electroacupuncture were detected.Results After the 7th day,body mass was significantly decreased(P<001)and fasting glucose was significantly increased(P<001)in the model group compared with the normal group.After the 21st day,compared with the model group,heat pain threshold of the model+electroacupuncture group was significantly higher(P<001);the results of co⁃expression of TRPV4 and NeuN immunofluorescence on rat L4~L6 DRG showed that:the expression of positive cells in the model group was significantly higher(P<001)than that in the normal group,the co⁃expression of TRPV4 and NeuN positive cells in L4~L6 DRG of rats in the model+electroacupuncture group was significantly lower(P<001)than that in the model group.The TRPV4 agonist GSK1016790A can reverse the downregulation of thermal pain threshold induced by electroacupuncture in DNP rats(P<001).Conclusion Electroacupuncture alleviated the DNP induced by STZ,and its mechanism may involve the inhibition of TRPV4 protein expression in the DRG.

关 键 词：糖尿病神经痛 电针 背根神经节 瞬时受体电位香草蛋白4 

分 类 号：Q95-33[生物学—动物学]