碱、旱胁迫对乌拉尔甘草种子萌发及幼苗生理特性的影响  

Effect of alkaline and drought stress on seed germination and seedling physiological characteristics of Glycyrrhiza uralensis

作　　者：张奥 张传领 薛焱 贾鑫 曹阳 王晓琴 ZHANG Ao;ZHANG Chuan-ling;XUE Yan;JIA Xin;CAO Yang;WANG Xiao-qin(Key Laboratory of Medicinal Plant Resources Development and Sustainable Utilization,Inner Mongolia Medical University,College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古医科大学药用植物资源开发与可持续利用重点实验室,内蒙古呼和浩特010110

出　　处：《草原与草坪》2025年第1期173-181,共9页Grassland and Turf

基　　金：内蒙古自治区自然科学基金项目(2021BS03046);内蒙古医科大学2022年度校级科研项目(YKD2022MS031);内蒙古医科大学2023年度大学生创新创业训练计划项目(S202310132007X)。

摘　　要：【目的】探讨碱、旱胁迫对乌拉尔甘草种子萌发和幼苗生长特性的影响,找出乌拉尔甘草对碱、旱胁迫的耐受阈值,以利于逆境条件下保障种子萌发和幼苗生长。【方法】设置不同浓度的Na_(2)CO_(3)、NaHCO_(3)两种碱性盐和PEG-6000处理乌拉尔甘草种子,测定发芽率、发芽势、发芽指数、活力指数、相对盐害率、萌发抗旱指数、胚根长、胚根宽及4种生理指标。【结果】30 mmol/L浓度的Na_(2)CO_(3)和NaHCO_(3)处理对乌拉尔甘草种子萌发具有一定的促进作用,45 mmol/L浓度2种碱性盐处理开始表现出盐害,所有碱性盐对发芽势均表现为抑制作用。5%浓度的PEG-6000处理对五个种子萌发指标均具有显著的促进作用。Na_(2)CO_(3)处理对甘草胚根伸长生长没有显著的促进作用,并会显著抑制胚根宽的生长。NaHCO_(3)处理对甘草胚根伸长生长和胚根宽生长均表现为显著的抑制作用。除14%和17%浓度的PEG-6000处理外,其他浓度对甘草幼苗的伸长生长均表现为显著的促进作用,所有浓度PEG-6000处理对甘草幼苗的胚根宽生长均无显著的促进作用,轻微的干旱胁迫有利于甘草种子萌发和幼苗生长。MDA含量与Na_(2)CO_(3)处理下甘草幼苗的胚轴长、胚根宽均具有极显著负相关关系,SOD活性与PEG-6000处理下甘草幼苗的胚轴长、胚根宽均具有极显著负相关关系。【结论】5%浓度的PEG-6000处理有利于乌拉尔甘草种子萌发和幼苗生长,乌拉尔甘草种子萌发和幼苗形态建成对Na_(2)CO_(3)、NaHCO_(3)和PEG-6000胁迫的耐受阈值分别小于45、45 mmol/L和8%。乌拉尔甘草幼苗体内MDA含量可作为评价耐受Na_(2)CO_(3)处理的生理指标,SOD活性可作为评价耐受PEG-6000的生理指标。【Objective】To investigate the effects of alkali and drought stress on the seed germination and seed⁃ling growth characteristics of Glycyrrhiza uralensis,and to determine the tolerance thresholds of Glycyrrhiza uralen⁃sis to these stresses,in order to facilitate seed germination and seedling growth under adverse conditions.【Method】Set different concentrations of Na_(2)CO_(3),NaHCO_(3),and PEG⁃6000 to treat Glycyrrhiza uralensis seeds,and measure germination rate,germination potential,germination index,vigor index,relative salt damage rate,germination drought resistance index radicle length,hypocotyl width,and four physiological indicators.【Result】Treatment with Na_(2)CO_(3)and NaHCO_(3)at a concentration of 30 mmol/L has a certain promoting effect on the germination of Glycyrrhiza ura⁃lensis seeds.At a concentration of 45 mmol/L,the treatment with both alkaline salts began to show salt damage,and all alkaline salts inhibited germination potential.Treatment with 5%PEG⁃6000 significantly improvedall five seed germination indicators.Na_(2)CO_(3)treatment did not significantly promote the elongation growth of Glycyrrhiza uralen⁃sis embryo roots and significantly inhibited hypocotyl width.NaHCO_(3)treatment showed significant inhibitory effects on both the elongation and width growth of Glycyrrhiza uralensis embryo roots.Except for treatments with 14%and 17%concentrations of PEG⁃6000,all other concentrations significantly promoted the elongation of Glycyrrhiza ura⁃lensis seedlings.However,noconcentration of PEG⁃6000 significantly promotedthe width growth of Glycyrrhiza ura⁃lensis seedlings.Mild drought stress positively affected Glycyrrhiza uralensis seed germination and seedling growth.The MDA content(malondialdehyde)is showed a significant negative correlation with hypocotyl length and embryo roots width of Glycyrrhiza uralensis seedlings under Na_(2)CO_(3)treatment,while SOD(superoxide dismutase)activity was negatively correlated with the length of hypocotyl and the width of embryo ro

关 键 词：乌拉尔甘草 碱胁迫 干旱胁迫 种子萌发 生理指标 

分 类 号：S567[农业科学—中草药栽培]