天山雪莲质膜水孔蛋白基因SiPIP1;3在番茄中的抗寒功能分析  

Functional analysis of the plasma membrane intrinsic protein gene SiPIP1;3 from Saussurea involucrata in tomato

作　　者：张晓丽 刘晓燕 夏雯雯[1] 李锦[1] ZHANG Xiao-Li;LIU Xiao-Yan;XIA Wen-Wen;LI Jin(Key Laboratory of Agricultural Biotechnology,College of Life Sciences,Shihezi University,Shihezi 832000,Xinjiang,China)

机构地区：[1]石河子大学生命科学学院农业生物技术重点实验室,新疆石河子832000

出　　处：《作物学报》2025年第4期863-872,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32260726);“天池英才”引进计划项目(CZ001617);石河子大学高层次人才科研启动项目(RCZK202470)资助。

摘　　要：水孔蛋白(Aquaporin,AQPs)作为细胞水分跨膜运输的主要通道蛋白,参与植物的生长及逆境适应过程。前期通过构建极端低温耐受植物天山雪莲(Saussurea involucrata)低温表达文库,获得质膜水孔蛋白基因SiPIP1;3。为分析SiPIP1;3基因在低温适应中的功能,构建植物表达载体并转化于冷敏感植物番茄。结果发现,SiPIP1;3基因表达通过促进番茄在低温处理下的可溶性蛋白、可溶性糖和脯氨酸积累,降低膜脂过氧化,从而显著增强番茄的冷耐受性;此外,大田种植结果表明,SiPIP1;3基因表达可通过提高番茄叶片气孔导度以增加胞间CO_(2)浓度,从而显著提高番茄净光合效率和水分利用效率,导致平均果实体积和平均单株果实数显著增加,显著提高单株番茄果实产量。因此,SiPIP1;3基因表达可显著增强番茄的抗寒性和提高番茄果实产量,为后期番茄抗寒新种质培育提供重要的基因资源和奠定理论基础。Aquaporins(AQPs),as key facilitators of water transport across cellular membranes,play an essential role in plant growth and adaptation to environmental stresses.Previously,the plasma membrane intrinsic protein gene SiPIP1;3 was cloned from a low-temperature expression library of Saussurea involucrata,a cold-tolerant herbaceous plant.To investigate the function of SiPIP1;3 under low-temperature stress,a plant expression vector containing SiPIP1;3 was constructed and transformed into cold-sensitive tomato plants.The results demonstrated that SiPIP1;3 expression significantly enhanced tomato tolerance to low-temperature treatment by promoting the accumulation of soluble proteins,soluble sugars,and proline,while reducing membrane lipid peroxidation.Moreover,field cultivation results revealed that SiPIP1;3 expression improved intercellular CO_(2) concentration by increasing stomatal conductance in tomato leaves.This led to a marked improvement in net photosynthetic efficiency and water use efficiency,ultimately resulting in a significant increase in both the average fruit size and fruit number per plant.In conclusion,the expression of SiPIP1;3 significantly enhances low-temperature tolerance and fruit yield in tomato plants.This study provides a valuable genetic resource and establishes a theoretical foundation for breeding cold-resistant tomato varieties.

关 键 词：SiPIP1 3基因 抗寒性 光合效率 果实产量 

分 类 号：S64[农业科学—蔬菜学]