基于种子发育转录组的豌豆bZIP基因家族分析及种子发育候选基因的鉴定  

Analysis of bZIP gene family and identification of seed development candidate genes in pea based on seed development transcriptome

作　　者：周恩强 缪亚梅[1] 周瑶 姚梦楠 赵娜[1] 王永强[1] 朱宇翔 薛冬 李宗迪 石宇欣 李波[1] 汪凯华[1] 顾春燕[1] 王学军[1] 魏利斌 ZHOU En-Qiang;MIAO Ya-Mei;ZHOU Yao;YAO Meng-Nan;ZHAO Na;WANG Yong-Qiang;ZHU Yu-Xiang;XUE Dong;LI Zong-Di;SHI Yu-Xin;LI Bo;WANG Kai-Hua;GU Chun-Yan;WANG Xue-Jun;WEI Li-Bin(Jiangsu Yanjiang Institute of Agricultural Sciences,Nantong 226012,Jiangsu,China)

机构地区：[1]江苏沿江地区农业科学研究所,江苏南通226012

出　　处：《作物学报》2025年第4期914-931,共18页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-08-Z10);江苏省农业科技自主创新项目[CX(22)2011];江苏省“双创人才”项目(YSSCRC2022469)资助。

摘　　要：bZIP基因广泛参与种子发育、光信号调节和胁迫响应等生理过程。然而,目前对豌豆PastbZIP基因调控种子发育知之甚少。为鉴定豌豆种子发育相关PastbZIP基因,揭示其进化关系,本研究利用生物信息学和种子发育转录组数据对PastbZIP基因家族进行鉴定与进化分析,并通过比较基因组学挖掘种子发育相关PastbZIP基因,同时利用蛋白互作网络和qRT-PCR对其验证。在豌豆种子发育转录组数据中共鉴定出62个PastbZIP基因,根据系统进化特征将PastbZIP基因分为9组,不同亚家族在蛋白理化性质、基因结构、保守基序等方面表现出进化多样性和差异性。共线性分析发现,豌豆与大豆、苜蓿、拟南芥和蚕豆分别有54、23、8和22对共线性基因,并且发现片段复制是PastbZIP基因家族扩张的主要驱动力,而且这些基因的进化是通过纯化选择来实现的。KEGG富集分析显示,PastbZIP主要富集在植物激素信号转导通路中,且PastbZIP启动子顺式作用元件中包含大量的激素响应元件,有60个(96.8%)PastbZIP基因包含5种激素响应元件。通过同源比对在豌豆中发现10个与种子发育相关的候选基因,且其蛋白互作基因均参与调控种子发育。综上所述,本研究首次对豌豆种子发育相关PastbZIP家族成员进行了鉴定和进化分析,并通过同源比对挖掘了种子发育候选基因,研究结果将为研究豌豆bZIP基因调控种子发育提供重要参考。The bZIP gene is widely involved in physiological processes such as seed development,light signal regulation,and stress response.However,little is known about the regulation of seed development by pea PastbZIP gene.In order to identify the PastbZIP gene family related to pea seed development and reveal its evolutionary relationship,this study used bioinformatics methods and seed development transcriptome data to identify and analyze the PastbZIP gene family,and mined the candidate PastbZIP genes related to seed development through comparative genomics,at the same time,it was verified by protein interaction network and qRT-PCR.A total of 62 PastbZIP genes were identified from the transcriptome data of pea seed development,and PastbZIP genes were divided into 9 groups according to phylogenetic characteristics,Different subfamilies showed evolutionary diversity and differences in protein physicochemical properties,gene structure,conserved motifs,etc.Collinearity analysis showed that pea had 54,23,8,and 22 pairs of collinearity gene with soybean,Medicago truncatula,Arabidopsis and faba bean,respectively.More important,it was found that the fragment replication was the main driving force for the expansion of the PastbZIP gene family,and the evolution of these genes was achieved through purification selection.KEGG enrichment analysis showed that PastbZIP was mainly enriched in plant hormone signal transduction pathways.The same is that the cis-acting elements of the PastbZIP promoter contain a large number of hormone response elements,and 60 PastbZIP(96.8%)genes contained five hormone response elements.Ten candidate genes related to seed development were found in pea by homologous comparison,and protein interaction genes also regulated seed development.In conclusion,this study was the first to identify and analyze the members of the PastbZIP family related to seed development in pea,and excavated the candidate genes related to seed development through homologous alignment.These results will provide important reference

关 键 词：豌豆 PastbZIP 基因家族 种子发育 进化分析 蛋白互作网络 

分 类 号：Q94[生物学—植物学]