不同地区柑橘根际土壤细菌群落结构及功能预测分析  

Analysis of Bacterial Community Structure and Functional Prediction in Citrus Rhizosphere Soils from Different Regions

作　　者：袁昭弟 蒋俊杰 胡礼川 段婷婷 杨俊 朱惠敏 张海鸿 张小夏 朱利君 付强 YUAN Zhaodi;JIANG Junjie;HU Lichuan;DUAN Tingting;YANG Jun;ZHU Huimin;ZHANG Haihong;ZHANG Xiaoxia;ZHU Lijun;FU Qiang(College of Life Science and Biotechnology,Mianyang Teachers'College,Mianyang Sichuan 621000,China;Liangshan Yi Autonomous Prefecture Academy of Agricultural Sciences,Liangshan Sichuan 615000,China)

机构地区：[1]绵阳师范学院生命科学与技术学院,四川绵阳621000 [2]凉山彝族自治州农业科学研究院,四川凉山615000

出　　处：《耕作与栽培》2025年第2期8-18,36,共12页Tillage and Cultivation

基　　金：国家现代农业产业技术体系四川蚕桑创新小组项目(SCCXTD-2024-17);四川省自然科学基金项目(4NSFSC0401);绵阳师范学院校级项目(QD2023A22)。

摘　　要：为探究不同地区柑橘园土壤细菌群落的结构、功能与养分可用性,对绵阳市3个采样点9个样品进行土壤理化分析,结合高通量测序技术比较河边镇(A组)、黄土镇(B组)和花荄镇(C组)柑橘根际土壤细菌群落多样性、结构及功能的差异,并挖掘不同地区的优势细菌,分析环境因素对细菌结构和功能的影响。结果表明,柑橘根际土壤细菌群落丰富度A组显著高于B组和C组,而A组细菌群落多样性最高,其次为B组,C组最低。主成分分析表明,不同地区柑橘根际土壤细菌群落结构具有明显差异;冗余分析表明,土壤有机碳和pH值是影响柑橘根际土壤细菌群落结构和功能的重要因子。在门水平上,A、B、C组对应的优势菌门分别为酸杆菌门(Acidobacteriota)、变形菌门(Proteobacteria)以及拟杆菌门(Bacteroidota)。属水平上,未鉴定细菌属Vicinamibacterales、Gemmatimonadaceae是A组的优势细菌属,朱氏杆菌属(Chujaibacter)和芽孢八叠球菌属(Sporosarcina)是B组的优势细菌属,而C组优势细菌属为鞘氨醇单胞菌属(Sphingomonas)和未鉴定细菌属Chitinophagaceae。根据PICRUST功能预测分析,土壤细菌的功能途径主要集中在新陈代谢、遗传信息处理和环境信息处理等方面。To investigate the impact of different regions on the structure,function,and nutrient availability of soil bacterial communities in citrus orchards,soil physicochemical analyses were conducted on nine samples from three sampling sites in Mianyang City.High-throughput sequencing technology was employed to compare the diversity,structure,and function of bacterial communities in the rhizosphere soil of citrus trees from Hebian Town(Group A),Huangtu Town(Group B),and Hua'ai Town(Group C).The dominant bacteria in different regions were identified,and the influence of environmental factors on bacterial structure and function was analyzed.The results showed that the richness of bacterial communities in the rhizosphere soil of citrus trees was significantly higher in Group A than in Groups B and Groups C,with Group A exhibiting the highest bacterial diversity,followed by Group B,and Group C showing the lowest.Principal component analysis revealed distinct differences in the structure of bacterial communities in the rhizosphere soil of citrus trees across different regions.Redundancy analysis demonstrated that soil organic carbon and pH were significant factors influencing the structure and function of bacterial communities in the rhizosphere soil of citrus trees.At the phylum level,the dominant bacterial phyla in Groups A,Groups B,and Groups C were Acidobacteriota,Proteobacteria,and Bacteroidota,respectively.At the genus level,the dominant bacterial genera in Group A were the unidentified genera Vicinamibacterales and Gemmatimonadaceae,while Chujaibacter and Sporosarcina were dominant in Group B,and Sphingomonas and the unidentified genus Chitinophagaceae were dominant in Group C.According to PICRUST functional prediction analysis,the functional pathways of soil bacteria were primarily concentrated in metabolism,genetic information processing,and environmental information processing.

关 键 词：柑橘 根际土壤 高通量测序 细菌群落结构 功能预测 土壤理化 

分 类 号：S666.29[农业科学—果树学]