迷迭香酸减轻分泌性中耳炎大鼠炎症反应和听力损伤的影响  

Rosmarinic Acid Alleviates Effects of Inflammatory Response and Hearing Impairment in Rats with Secretory Otitis Media

作　　者：胡成莉 康厚墉 赵冉 HU Chengli;KANG Houyong;ZHAO Ran(Chongqing Yubei District People's Hospital,Chongqing 401120,China;不详)

机构地区：[1]重庆市渝北区人民医院,重庆401120 [2]重庆医科大学附属第一医院

出　　处：《中华耳科学杂志》2025年第2期267-272,共6页Chinese Journal of Otology

基　　金：重庆市中医药科技计划项目(ZY201802143)。

摘　　要：目的探讨迷迭香酸对分泌性中耳炎(secretory otitis media,SOM)大鼠炎症反应和听力损伤的影响及白细胞介素(interleukin,IL)-6/转录信号转导子与激活子3(signal transducers and activators of transcription 3,STAT3)信号通路的作用。方法选取健康雄性SD大鼠72只,通过卵清蛋白诱导SOM大鼠模型,将建模成功的大鼠分为模型组、迷迭香酸-L、M、H组、迷迭香酸+Colivelin组,并设置假手术组,每组12只。药物干预结束后,耳内镜观察鼓膜形态;酶联免疫吸附法检测大鼠血清中炎症相关因子IL-6、肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、IL-1β水平及中耳灌洗液中IL-6水平,苏木精-伊红染色观察大鼠中耳黏膜组织病理学变化;实时荧光定量逆转录聚合酶链反应检测大鼠中耳黏膜组织中IL-6/STAT3通路相关基因水平;Western blot检测大鼠中耳黏膜组织中IL-6/STAT3通路相关蛋白水平。结果与假手术组比较,模型组大鼠鼓膜有大量积液、明显的放射性血管、中耳黏膜层厚度增加、上皮细胞坏死、有大量炎症细胞浸润、血清中炎症相关因子IL-6、TNF-α、IL-1β水平、中耳灌洗液中IL-6水平、中耳黏膜组织IL-6、STAT3的mRNA水平、p-STAT3/STAT3、IL-6、JAK2蛋白水平显著升高,差异均有统计学意义(P<0.05);与模型组比较,迷迭香酸-L、M、H组大鼠鼓膜恢复透明、结构逐渐清晰、积液减少、中耳黏膜组织结构逐渐恢复、炎症细胞浸润减轻、血清中炎症相关因子IL-6、TNF-α、IL-1β水平及中耳灌洗液中IL-6水平、中耳黏膜组织IL-6、STAT3的mRNA水平、p-STAT3/STAT3、IL-6、JAK2蛋白水平显著降低,差异均有统计学意义(P<0.05);与迷迭香酸-H组比较,迷迭香酸+Colivelin组大鼠鼓膜颜色变暗、有大量积液、结构模糊、有明显的放射性血管、中耳黏膜层厚度增加、上皮细胞坏死、有大量炎症细胞浸润、血清中炎症相关因子IL-6、TNF-α、IL-1β水平及中耳灌洗液Objective To investigate the impacts of rosmarinic acid on inflammatory response and hearing loss in rats with secretory otitis media(SOM)and the role of interleukin(IL)-6/signal transducers and activators of transcription 3(STAT3)signaling pathway.Methods Select 72 healthy male SD rats,SOM in rats was induced by ovalbumin(OVA).Rats with SOM received rosmarine-L,M,H,or rosmarinic acid+Colivelin treatments,or no treatment(n=12 in each group).A sham operation group was also included(n=12).After drug intervention,endoscopy was used to examine the tympanic membrane;ELISA was used to detect the levels of inflammatory related factors IL-6,tumor necrosis factor-α(TNF-α),IL-1β in rat serum and IL-6 in middle ear lavage;HE staining was used to observe histopathological changes in middle ear mucosa;RT-qPCR was used to detect the levels of IL-6/STAT3 pathway related genes in middle ear mucosa;Western blot was used to detect the levels of IL-6/STAT3 pathway related proteins in rat middle ear mucosa.Results Compared with the sham operation group,untreated rats with SOM showed a large amount of effusion,engorged blood vessels on the tympanic membrane,increased middle ear mucosa thickness,epithelial necrosis,a large number of inflammatory cell infiltration,and significantly increased levels of inflammation related factors IL-6,TNF-α,IL-1β in serum,IL-6 in middle ear lavage,mRNA levels of IL-6,STAT3 in middle ear mucosal,and protein levels of p-STAT3/STAT3,IL-6,and JAK2(P<0.05).In contrast,animals treated with rosmarinic acid L,M,and H showed a return to transparent tympanic membrane,with clear structural landmarks,reduced fluid accumulation,gradual restoration of middle ear mucosal structure,and reduced inflammatory cell infiltration,with significantly reduced levels of inflammation related factors IL-6,TNF-α,IL-1βin serum,IL-6 in middle ear lavage,mRNA levels of IL-6,STAT3 in middle ear mucosal,and protein levels of p-STAT3/STAT3,IL-6,and JAK2(P<0.05).Compared with the rosmarinic acid-H treated group,rats in the rosmari

关 键 词：迷迭香酸 IL-6/STAT3信号通路 分泌性中耳炎 大鼠 听力损伤 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]