鸡与鼠耳蜗毛细胞损伤后再生修复的研究进展  

Progress in the regeneration of cochlear hair cells after damage in chickens and mice

作　　者：张信文 杨思远[1,3] 江凌玲 白焕菊 曾少举 ZHANG Xin-Wen;YANG Si-Yuan;JIANG Ling-Ling;BAI Huan-Ju;ZENG Shao-Ju(College of Pharmacy,Hainan Vocational University of Science and Technology,Haikou 571126,China;College of Life Science,Hainan Normal University,Beijing 100875,China;Beijing Key Laboratory of Gene Resource and Molecular Development,Beijing Normal University,Haikou 571158,China;Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;The High School Affiliated to Renmin University of China,Beijing 100080,China)

机构地区：[1]海南科技职业大学医药学院,海口571126 [2]海南师范大学生命科学学院,北京100875 [3]北京师范大学基因资源与分子发育北京市重点实验室,海口571158 [4]首都医科大学附属北京天坛医院,北京100070 [5]中国人民大学附属中学,北京100080

出　　处：《生命科学》2025年第2期206-211,共6页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金项目(31860269);海南省自然科学基金项目(319QN238)。

摘　　要：耳毒性药物等造成的毛细胞损伤脱落可导致人们的严重听力障碍。研究显示,在非哺乳动物中,鸡的胚胎期毛细胞具有一定抵抗耳毒性药物损伤的能力,出生后毛细胞受损后,能自我修复到损伤前状态。耳蜗结构、发育及生理功能在哺乳类与非哺乳类间高度保守同源,找出参与鸡耳蜗毛细胞受损后自我修复基因,比较这些基因在小鼠耳蜗受损后对应时段中的差异,通过腺病毒作基因载体,转染这些差异基因到受损后的鼠耳蜗上皮细胞,改变它们的表达活性,可一定程度实现小鼠毛细胞的再生。以上工作为通过基因治疗手段,提高哺乳动物毛细胞损伤后的再生修复提供了新策略和契机。Ototoxic drugs and other damage can cause hair cell apoptosis and loss,resulting in serious deafness.It has been shown that chick hair cells in some embryonic stages are prevented from injury from ototoxic drugs to some degree,whereas hair cells in post-hatching can be regenerated after injury and return to the state before injury.The structure,development and physiological functions of cochlea are highly conserved and homologous between mammals and non-mammals.After the genes involved in the hair cell regeneration after injury are determined in the chick,they are compared with those in the mouse hair cells in the corresponding stages after injury.Some of these genes expressed differently in the chick or mouse are constructed into the adenoviruses,and transfected into epithelium cells in the cochlea of mice after damage by aminoglycoside antibiotic to change their expressing activities.The results indicate that some regenerated cells are obtained in the cochlea of mice after damage.These results provide a new strategy and chance to increase cell proliferation and cell differentiation towards hair cells in the cochlea of mice after damage.

关 键 词：耳蜗损伤 听感觉上皮 毛细胞再生 鸡 小鼠 氨基糖苷类抗生素 

分 类 号：Q28[生物学—细胞生物学] R764.3[医药卫生—耳鼻咽喉科]