3种食用菌废菌棒生物炭的特性及其吸附巨大芽孢杆菌的性能  

Characterization and Performance in Adsorption of Bacillus megaterium of Biochar from Three Edible Fungus Spent Artificial Logs

作　　者：杨洁[1] 王思凡 张铭栋 袁宗胜 YANG Jie;WANG Sifan;ZHANG Mingdong;YUAN Zongsheng(College of Environment and Safety Engineering,Fuzhou University,Fuzhou 350108,China;College of Future Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Geography and Oceanography/Fuzhou Institute of Oceanography,Minjiang University,Fuzhou 350108,China)

机构地区：[1]福州大学环境与安全工程学院,福建福州350108 [2]福建农林大学未来技术学院,福建福州350002 [3]闽江学院地理与海洋学院/福州海洋研究院,福建福州350108

出　　处：《云南农业大学学报(自然科学版)》2025年第1期151-158,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：福州海洋研究院科技项目(2022F06);福建省林业科技项目(2023FKJ12)。

摘　　要：【目的】探究不同食用菌废菌棒生物炭的理化特性及其吸附巨大芽孢杆菌(Bacillus megaterium)的能力,为食用菌废菌棒的高值化利用提供依据。【方法】以3种食用菌(金针菇、真姬菇、银耳)废菌棒为原料,在700℃、CO_(2)或N_(2)氛围下热解2 h制成6种生物炭,对其理化性质进行表征,并通过正交试验研究生物炭对巨大芽孢杆菌的吸附性能。【结果】6种生物炭均呈碱性,产率范围在34%~42%之间,灰分含量在10%~59%之间,碳含量在44%~72%之间;芳香化程度高,均具有利于微生物吸附的官能团;平均孔径为1.92~4.94 nm,孔体积为0.012~0.099 cm^(3)/g,且CO_(2)氛围下热解制备的生物炭,其比表面积和孔体积均大于N_(2)氛围下热解制备的生物炭;CO_(2)氛围下制备的生物炭,其吸附巨大芽孢杆菌的最优条件组合为温度25℃、菌炭比10∶1、吸附时间24 h,吸附率均大于93%。【结论】以金针菇废菌棒为原料,在CO_(2)氛围下制备的生物炭,其吸附效果最好,吸附率达95.55%,具有作为载体及进一步应用的潜力。研究结果为食用菌废菌棒的资源化和高值化利用提供了新途径。[Purpose]To investigate the physicochemical properties of biochars made from different edible fungus spent artificial logs and their ability to adsorb Bacillus megaterium,providing a basis for the high-value utilization of edible fungus spent artificial logs.[Methods]Three types of edible fungus(Flammulina velutipes,Hypsizygus marmoreus,and Tremella fuciformis)spent artificial logs were used as raw materials,six types of biochars were produced by pyrolysis for two hours at 700℃under CO_(2) or N_(2) atmosphere.The biochars were characterized for their physicochemical prop erties,and the adsorption performance of biochars for B.megaterium was studied through an orthogonal experiment.[Results]All six types of biochars were alkaline,with yields ranging from 34%to 42%,ash content ranging from 10%and 59%,and carbon content ranging from 44%and 72%.The biochars had high aromaticity and functional groups beneficial to microbial adsorption.The average pore size ranged from 1.92 to 4.94 nm,and the pore volume ranged from 0.012 to 0.099 cm^(3)/g.Biochars obtained under CO_(2) atmosphere had a higher specific surface area and pore volume compared to that obtained under N_(2) atmosphere.The optimal combination of temperature,fungal suspension-to-biochar ratio,and adsorption time for adsorption of B.megaterium by the biochars derived from CO_(2) atmosphere were 25℃,10∶1,and 24 hours,respectively,with adsorption rates above 93%.[Conclusion]The biochar made from F.velutipes spent artificial log under CO_(2) atmosphere have the best adsorption effect,with an adsorption rate of 95.55%,demonstrating its potential as a carrier and for further applications.The results provide a new approach for the resource utilization and high-value application of edible fungus spent artificial logs.

关 键 词：生物炭 食用菌废菌棒 表征 热解氛围 微生物吸附 

分 类 号：S646[农业科学—蔬菜学]