冠心病气虚血瘀证舌苔菌群特征分析  

Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome

作　　者：王楚皓 刘永月 丁照烜 张晓晴 WANG Chuhao;LIU Yongyue;DING Zhaoxuan;ZHANG Xiaoqing(School of Life Science,Beijing University of Chinese Medicine,Beijing,100029)

机构地区：[1]北京中医药大学生命科学学院,北京市100029

出　　处：《中医杂志》2025年第5期501-508,共8页Journal of Traditional Chinese Medicine

基　　金：国家重点研发计划(2022YFC3502301);国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023263)。

摘　　要：目的探究冠心病气虚血瘀证舌苔菌群特征。方法纳入冠心病气虚血瘀证患者27例组成气虚血瘀证组,非气虚血瘀证冠心病患者29例组成非气虚血瘀证组,健康人20例组成健康组。使用16S rDNA高通量测序技术检测受试者舌苔菌群,并进行Alpha、Beta多样性分析和菌群丰度比较。结果共检测出479条操作分类单元(OTU),其中245条OTU为三组共有,气虚血瘀证组特有OTU 33条,非气虚血瘀证组特有OTU 21条,健康组特有OTU 121条。气虚血瘀证组、非气虚血瘀证组受试者舌苔菌群观测物种数(Sob)、菌群物种总数(Chao1)、丰富度覆盖指数(ACE)、香浓指数(Shannon)较健康组减少(P<0.05)。三组间舌苔菌群PCoA分析距离矩阵差异具有统计学意义(P<0.05)。与健康组比较,气虚血瘀证组受试者舌苔放线菌门、帕氏菌门、螺旋体菌门、疣微菌门、罗氏菌属、TM7X、珍珠链菌属、棒状杆菌属丰度增加,梭杆菌门、蓝细菌门、细长丝菌属、乳酸菌属丰度减少(P<0.05);非气虚血瘀证组受试者舌苔放线菌门、疣微菌门、罗氏菌属、棒状杆菌属丰度增加,蓝细菌门、乳酸菌属丰度减少(P<0.05)。与非气虚血瘀证组比较,气虚血瘀证组受试者舌苔帕氏菌门、消化链球菌属、索罗杆菌属、丝状菌属、莫雷梭菌、牙髓卟啉单胞菌、龈沟产线菌的丰度显著增加,蓝细菌门比例减少(P<0.05)。结论冠心病气虚血瘀证患者呈现有益菌减少,致病菌增加的特点,帕氏菌门、消化链球菌属、索罗杆菌属、丝状菌属、莫雷梭菌、牙髓卟啉单胞菌、龈沟产线菌是其与非气虚血瘀证的差异菌群。Objective To explore the characteristics of the tongue coating microbiota in patients of coronary heart disease(CHD)with qi deficiency and blood stasis syndrome.Methods A total of 27 CHD patients with qi deficiency and blood stasis syndrome,29 patients with non-qi deficiency and blood stasis syndrome,and 20 healthy individuals were included in this study.The tongue coating microbiota of the participants was analyzed using 16S rDNA high-throughput sequencing technology,followed by Alpha and Beta diversity analyses and comparisons of microbial abundance.Results A total of 479 operational taxonomic units(OTUs)were detected,among which 245 OTUs were shared across all three groups.There were 33 OTUs unique to the qi deficiency and blood stasis syndrome group,21 OTUs unique to the non-qi deficiency and blood stasis syndrome group,and 121 OTUs unique to the healthy group.The observed species count(Sob),total species richness(Chao1),abundance-based coverage estimator(ACE),and Shannon diversity index were significantly lower in the qi deficiency and blood stasis syndrome and non-qi deficiency and blood stasis syndrome groups compared to the healthy group(P<0.05).Principal coordinate analysis(PCoA)of the tongue coating microbiota showed significant differences in distance matrices among the three groups(P<0.05).Compared with the healthy group,the qi deficiency and blood stasis syndrome group exhibited an increased abundance of Actinobacteria,Patescibacteria,Spirochaetes,Verrucomicrobia,Rothia,TM7X,Gemella,and Corynebacterium,while Fusobacteria,Cyanobacteria,Leptotrichia,and Lactobacillus decreased(P<0.05).In the non-qi deficiency and blood stasis syndrome group,Actinobacteria,Verrucomicrobia,Rothia,and Corynebacterium increased,whereas Cyanobacteria and Lactobacillus reduced(P<0.05).When comparing with the non-qi deficiency and blood stasis syndrome group,the qi deficiency and blood stasis syndrome group had a significantly higher abundance of Patescibacteria,Peptostreptococcus,Solobacterium,Filifactor,Moraxella,Porphyromo

关 键 词：冠心病 气虚血瘀证 舌苔菌群 

分 类 号：R259[医药卫生—中西医结合]