北京市顺义区白纹伊蚊Brevihamaparvovirus属病毒的分离与鉴定  

Isolation and identification of Aedes albopictus Densovirus(Brevihamaparvovirus)in Shunyi District,Beijing,China

作　　者：许秀燕 阎婷 李静[1] 朱思捷[2] 张洪江[1] 周小洁[1] 佟颖[1] 张勇[1] XU Xiu-yan;YAN Ting;LI Jing;ZHU Si-jie;ZHANG Hong-jiang;ZHOU Xiao-jie;TONG Ying;ZHANG Yong(Institute of Disinfection and Vector Control,Beijing Center for Disease Prevention and Control,Beijing 100013,China;Beijing Shunyi District Center for Disease Prevention and Control,Beijing 101300,China)

机构地区：[1]北京市疾病预防控制中心消毒与有害生物防制所,北京100013 [2]北京市顺义区疾病预防控制中心,北京101300

出　　处：《中国媒介生物学及控制杂志》2025年第1期4-10,共7页Chinese Journal of Vector Biology and Control

基　　金：首都卫生发展科研专项(2024-2G-30116)。

摘　　要：目的对2021年北京市顺义区采集的白纹伊蚊标本进行病毒分离和鉴定。方法将蚊虫研磨液平行接种叙利亚仓鼠肾细胞(BHK-21细胞)和白纹伊蚊卵细胞(C6/36细胞)进行病毒分离,对病毒分离物开展分子生物学鉴定。结果2021年在北京市顺义区野外采集的白纹伊蚊标本中分离到1株可以引起C6/36细胞病变的病毒分离物(AalDV-8)。病毒基因组编码区核苷酸序列的分析结果显示,AalDV-8病毒为单链DNA病毒,该病毒基因组编码区全长为3335 nt,共编码2个非结构蛋白(NS1、NS2)和1个衣壳蛋白(VP),3种蛋白的核苷酸(氨基酸)序列长度分别为2376 nt(791 aa)、1092 nt(363 aa)、1071 nt(356 aa)。经病毒系统进化分析发现,AalDV-8病毒位于Brevihamaparvovirus属病毒进化组,并与中国广州市白纹伊蚊中分离的AalDV-7病毒亲缘关系最近。结论首次从北京市白纹伊蚊野外种群中分离出Brevihamaparvovirus属病毒。Objective To isolate and identify viruses in Aedes albopictus specimens from Shunyi District,Beijing,China in 2021.Methods The virus was isolated by parallel inoculation of Syrian hamster kidney cell line BHK-21 and Ae.albopictus C6/36 cells with mosquito grinding liquid,and molecular biological identification was carried out for the virus isolates.Results A virus isolate(AalDV-8)was isolated from Ae.albopictus specimens collected from the field in Shunyi District,Beijing in 2021,which could cause C6/36 cell lesion.The nucleotide sequence analysis of the virus genome coding region showed that AalDV-8 virus was a single-stranded DNA virus with a total length of 3335 nt,encoding 2 non-structural proteins(NS1 and NS2)and 1 capsid protein(VP).The nucleotide(amino acid)sequence lengths of the three proteins were 2376 nt(791 aa),1092 nt(363 aa),and 1071 nt(356 aa),respectively.The phylogenetic analysis showed that AalDV-8 virus belonged to Brevihamaparvovirus group and was closely related to AalDV-7 virus isolated from Ae.albopictus in Guangzhou,China.Conclusion This is the first time to isolate Brevihamaparvovirus from the field Ae.albopictus collected from in Beijing.

关 键 词：细小病毒 Brevihamaparvovirus属 白纹伊蚊 

分 类 号：R373.3[医药卫生—病原生物学] S476.13[医药卫生—基础医学]