基于DNA条形码的八门湾尼蠓雄虫的发现与描述(双翅目:蠓科)  

Discovery and description of male Nilobezzia bamenwana based on DNA barcodes(Diptera:Ceratopogonidae)

作　　者：杨浩 娄嘉雯 林晓丹 李加慧 YANG Hao;LOU Jia-wen;LIN Xiao-dan;LI Jia-hui(Key Laboratory of Green Prevention and Control of`Tropical Plant Diseases and Pests,Ministry of Education,School of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带农林学院,热带农林生物灾害绿色防控教育部重点实验室,海南海口570228

出　　处：《中国媒介生物学及控制杂志》2025年第1期94-98,共5页Chinese Journal of Vector Biology and Control

基　　金：海南省自然科学基金高层次人才项目(421RC485);海南省自然科学基金青年项目(320QN201);中科协第八届青年人才托举工程项目(2022QNRC001);海南大学科研启动项目[KYQD(ZR)20026]。

摘　　要：目的利用DNA条形码技术对采自海口市的6只尼蠓雄虫进行物种鉴定并进行形态学描述。方法利用无损形态基因组DNA提取法提取6头尼蠓雄虫的基因组DNA并制作玻片标本,针对细胞色素C氧化酶亚基Ⅰ基因PCR扩增并测序,通过美国国立生物技术信息中心数据库的基于局部比对算法的搜索工具(BLAST)采用邻接法进行序列比对和构建系统发育树进行物种鉴定,对主要鉴定特征进行拍摄、测量和绘图。结果6只尼蠓雄虫的DNA条形码序列(序列登录号:PP351822~PP351827)与八门湾尼蠓(Nilobezzia bamenwana Li&Li,2019)雌性模式标本的序列相似度介于97.72%~99.85%,构建的系统发育树显示,6条待鉴定的尼蠓雄虫序列与3条八门湾尼蠓雌虫序列聚为一支,支持率达到100%,6头尼蠓雄虫均为八门湾尼蠓,其主要鉴别特征为:小盾片粗鬃9根;中足股节,后足股节、胫节、跗节具明显的粗长鬃毛;第9背板中部具一深色角质化“V”形带,抱握器基节与端节愈合,整体呈直棒状,阳基侧突全愈合。新发现雄虫标本保存于海南大学热带农林学院昆虫标本馆。结论通过DNA条形码技术首次鉴定发现了八门湾尼蠓的雄虫,并对其形态特征进行了描述。Objective To identify and morphologically characterize six Nilobezzia males collected from Haikou,China using DNA barcoding.Methods A morphologically non-invasive method was used to extract genomic DNAs from the six Nilobezzia males.Slide specimens were made subsequently,and the cytochrome c oxidase subunitⅠ(COⅠ)gene was subjected to polymerase chain reaction(PCR)amplification and sequencing.Species identification was performed using the basic local alignment search tool(BLAST)by aligning sequences with the National Center for Biotechnology Information database and constructing a phylogentic tree based on neighbor-joining method.Major morphological features were photographed,measured,and sketched.Results DNA barcodes of the six Nilobezzia males were sequenced(sequence accession:PP351822-PP351827),showing a high sequence similarity(ranging from 97.72%to 99.85%)with the specimens of N.bamenwana females.The constructed neighbor-joining tree indicated that the six sequences of Nilobezzia males and three sequences of N.bamenwana were clustered into one branch,with a support rate of 100%.Therefore,the six Nilobezzia males were identified as N.bamenwana.Morphological features included:scutellum with 9 large setae,mid-and hind-foot thighs,tibiae,and tarsomeres with obviously long bristles,tergiteⅨwith a sclerotizedⅤ-shaped area,gonostylus straight and parameres completely fused.The slide specimens of the newly identified males were kept in the Insect Collection of the School of Tropical Agriculture and Forestry,Hainan University.Conclusion For the first time,N.bamenwana males have been identified using DNA barcoding techniques and their morphological features have been characterized.

关 键 词：海南岛 DNA条形码 尼蠓属 捕食蠓 分类 

分 类 号：Q969.442.7[生物学—昆虫学]