电针翼腭区对变应性鼻炎大鼠NLRP3介导的细胞焦亡和炎性因子的影响  

Effects of electroacupuncture at pterygopalatine region on NLRP3-mediated pyroptosis and inflammatory factors in allergic rhinitis rats

作　　者：吕海洋 田美慧 佘书仪 刘昱成 孙磊 宋伍 汤勇 LV Haiyang;TIAN Meihui;SHE Shuyi;LIU Yucheng;SUN Lei;SONG Wi;TANG Yong(Changchun University of CM,Changchun 130117,Jilin Province,China;Third Clinical Hospital Affiliated to Changchun University of CM;Clinical College of Changchun University of CM,Changchun 130117,Jilin Province;Affiliated Hospital of Changchun University of CM,Changchun 130021,Jilin Province)

机构地区：[1]长春中医药大学,吉林长春130117 [2]长春中医药大学附属第三临床医院 [3]长春中医药大学临床医学院,吉林长春130117 [4]长春中医药大学附属医院,吉林长春130021

出　　处：《中国针灸》2025年第3期345-350,369,共7页Chinese Acupuncture & Moxibustion

基　　金：吉林省自然科学基金项目:20210101194JC;吉林省科学技术厅项目:20220203121SF;吉林省大学生创新创业计划项目:S202310199062。

摘　　要：目的:观察电针翼腭区对变应性鼻炎(AR)大鼠NOD样受体热蛋白结构域相关蛋白3(NLRP3)介导的细胞焦亡和炎性因子的影响。方法:将24只SD大鼠随机分为空白组、模型组、针刺组、电针组,每组6只。除空白组外,余组大鼠均经卵清白蛋白(OVA)致敏法建立AR大鼠模型。电针组大鼠予双侧翼腭区电针治疗,选择疏密波,频率2 Hz/100 Hz,电流0.5~1 mA,每次15 min,隔日1次,共3次;针刺组大鼠仅进行双侧翼腭区针刺,不连接电针。观察各组大鼠的鼻炎症状积分,HE染色观察大鼠鼻黏膜组织形态,ELISA法检测大鼠血清OVA特异性免疫球蛋白E(OVA-s Ig E)、白细胞介素(IL)-4、IL-6和IL-1β的含量,实时荧光定量PCR法检测鼻黏膜中NLRP3、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶-1(caspase-1)、IL-18的mRNA表达,Western blot法检测大鼠鼻黏膜中NLRP3、ASC、caspase-1、IL-18的蛋白表达。结果:与空白组比较,模型组大鼠鼻炎症状积分升高(P<0.01),血清OVA-sIgE、IL-4、IL-6、IL-1β含量均升高(P<0.05),鼻黏膜呈炎性浸润病理表现,且鼻黏膜组织中的NLRP3、ASC、caspase-1、IL-18的mRNA及蛋白表达升高(P<0.05);与模型组比较,电针组大鼠鼻炎症状积分降低(P<0.01),鼻黏膜病理表现改善,血清OVA-s Ig E、IL-4、IL-6和IL-1β的含量降低(P<0.05),鼻黏膜中的NLRP3、ASC、caspase-1、IL-18m RNA及蛋白表达降低(P<0.05)。结论:电针翼腭区可通过抑制NLRP3介导的细胞焦亡和炎性因子失衡来发挥抗炎作用,从而缓解AR大鼠的鼻炎症状。Objective To observe the effects of electroacupuncture at the pterygopalatine region on nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)-mediated pyroptosis and inflammatory factors in rats with allergic rhinitis(AR).Methods Twenty-four SD rats were randomly divided into a blank group,a model group,an acupuncture group and an electroacupuncture group,6 rats in each group.Except for the blank group,OVA-induced AR model was established in the remaining groups.In the electroacupuncture group,the rats were treated with electroacupuncture at the bilateral pterygopalatine region,with disperse-dense wave,in frequency of 2 Hz/100 Hz and current of 0.5-1 mA,15 min each time,once every other day,for 3 times.In the acupuncture group,the rats were treated with acupuncture at bilateral pterygopalatine region simply,without electrical stimulation.The rhinitis symptom score was observed,the pathomorphology of the nasal mucosa was observed by HE staining;the serum levels of OVA-specific immunoglobulin E(OVA-sIgE),interleukin(IL)-4,IL-6 and IL-1βwere detected by ELISA;the mRNA expression of NLRP3,apoptosis-associated speck-like protein containing CARD(ASC),cysteinyl aspartate specific proteinase-1(caspase-1)and IL-18 in the nasal mucosa was detected by real-time PCR;the protein expression of NLRP3,ASC,caspase-1 and IL-18 in the nasal mucosa was detected by Western blot.Results Compared with the blank group,in the model group,the rhinitis symptom score was increased(P<0.01),the serum levels of OVA-sIgE,IL-4,IL-6 and IL-1βwere increased(P<0.05),the nasal mucosa showed pathomorphology of inflammatory infiltration;the mRNA and protein expression of NLRP3,ASC,caspase-1 and IL-18 in the nasal mucosa was increased(P<0.05).Compared with the model group,in the electroacupuncture group,the rhinitis symptom score was reduced(P<0.01),the pathology of the nasal mucosa was improved;the serum levels of OVA-sIgE,IL-4,IL-6 and IL-1βwere decreased(P<0.05);the mRNA and protein expression of NLRP3,ASC,caspase-1 and IL-18 i

关 键 词：变应性鼻炎 电针 翼腭窝 NLRP3炎性小体 细胞焦亡 

分 类 号：R245.97[医药卫生—针灸推拿学]