固定化微生物IKP1对土霉素废水降解特性研究  

Study on degradation characteristics of oxytetracycline wastewater by immobilized microorganism IKP1

作　　者：卢航 钟为章[1,2] 宁志芳 刘春 牛建瑞[1,2] LU Hang;ZHONG Weizhang;NING Zhifang;LIU Chun;NIU Jianrui(College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;Pollution Prevention Biotechnology Laboratory of Hebei Province,Shijiazhuang 050018,China)

机构地区：[1]河北科技大学环境科学与工程学院,河北石家庄050018 [2]河北省污染防治生物技术实验室,河北石家庄050018

出　　处：《应用化工》2025年第2期362-366,374,共6页Applied Chemical Industry

基　　金：河北省高层次人才资助项目(A202101100);中央引导地方科技发展资金项目(236Z3811G)。

摘　　要：将一种高效降解土霉素(OTC)的克雷伯氏菌(Klebsiella sp.)采用吸附法固定在玉米秸秆生物炭上,研究了固定化细菌降解土霉素的效能及途径。结果表明,在投加量0.3 g、pH值7.0、温度35℃、外加2 g/L胰蛋白胨和处理时间3 d时,固定化菌剂对50 mg/L土霉素的降解率达到85.0%。经过3次循环实验后,仍能降解62.2%的土霉素,具有较高的回收利用率。固定化细菌通过吸附和生物降解两种机制去除土霉素,其中,生物降解在固定化系统中起主导作用(87%),并且土霉素经过降解后其生理毒性显著降低。此外,提出了新的土霉素生物降解途径,降解过程中主要发生了脱水、脱氨基、脱甲基、羟基化等。Klebsiella sp.,which can degrade oxytetracycline(OTC)efficiently,was fixed on corn stalk biochar by adsorption method.The efficiency and pathway of oxytetracycline degradation by immobilized bacteria were studied.The results showed that the degradation rate of 50 mg/L oxytetracycin was 85.0%when the dosage was 0.3 g,pH value was 7.0,temperature was 35℃,2 g/L tryptone was added and treatment time was 3 d.After three cycles of experiment,62.2%of oxytetracycline can still be degraded,which has a high recovery rate.The immobilized bacteria removed oxytetracycline by adsorption and biodegradation,in which biodegradation played a dominant role in the immobilized system(87%),and the physiological toxicity of oxytetracycline was significantly reduced after degradation.In addition,a new biodegradation pathway of oxytetracycline was proposed,in which dehydration,deamination,demethylation and hydroxylation were mainly involved.

关 键 词：土霉素 生物炭 固定化微生物 降解产物 降解途径 

分 类 号：TQ09[化学工程] X5[环境科学与工程—环境工程]