云南野独活的化学成分研究  

Chemical constituents from Miliusa tenuistipitata

作　　者：刘志莹 葛格 刘庭亮 郑文琳 郭亚萍 王书云 吴炎 LIU Zhi-ying;GE Ge;LIU Ting-liang;ZHENG Wen-lin;GUO Ya-ping;WANG Shu-yun;WU Yan(School of Pharmacy,Henan University,Kaifeng 475004,China;College of Pharmacy,Shenzhen Technology University,Shenzhen 518118,China)

机构地区：[1]河南大学药学院,河南开封475004 [2]深圳技术大学药学院,广东深圳518118

出　　处：《药学学报》2025年第3期646-654,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82004061)。

摘　　要：采用硅胶柱色谱、LH-20葡聚糖凝胶柱色谱及半制备液相等色谱分离技术,从云南野独活(Miliusa tenuistipitata W.T.Wang)枝叶的95%乙醇提取物中分离得到20个化合物。通过多种现代波谱学方法鉴定化合物结构,分别为tenuistone L(1)、tenuistone K(2)、樱花素(3)、藿香黄酮醇(4)、雷杜辛黄酮醇(5)、垂叶黄素(6)、蔓荆子黄素(7)、3,7-二甲氧基-5,3′,4′-三羟基黄酮(8)、泽兰黄醇(9)、(+)-miliusol(10)、miliusane XIX(11)、miliusane XVIII(12)、miliusolide(13)、19,20-dihydromiliusolide(14)、debilisoneC(15)、goniothalamusin(16)、2,10-dihydroxy-3,9-dimethoxy-8-oxo-protoberberine(17)、consanguine B(18)、6-羟基豆甾-4,22-二烯-3-酮(19)和6-羟基豆甾-4-烯-3-酮(20),其中化合物1和2为新化合物,化合物6、9、18和20为首次从番荔枝科植物中分离得到,化合物5、7、17和19为首次从野独活属植物中分离得到。采用MTT法进行抗肿瘤活性测试,结果发现化合物10和11可以显著抑制MDAMB-231和BT-549细胞增殖,其半数抑制浓度(IC50)为0.98~4.85μmol·L-1。Twenty compounds were isolated from the twigs and leaves of the 95%ethanol extract of Miliusa tenuistipitata W.T.Wang by various chromatographic techniques including silica gel column chromatography,sephadex LH-20 and preparative HPLC.Their structures were determined by analysis of spectral data as tenuistone L(1),tenuistone K(2),sakuranetin(3),pachypodol(4),retusin(5),penduletin(6),casticine(7),3,7-dimetoxi-5,3′,4′-tri-hidroxiflavona(8),eupatin(9),(+)-miliusol(10),miliusane XIX(11),miliusane XVIII(12),miliusolide(13),19,20-dihydromiliusolide(14),debilisone C(15),goniothalamusin(16),2,10-dihydroxy-3,9-dimethoxy-8-oxoprotoberberine(17),consanguine B(18),6β-hydroxystigmasta-4,22-dien-3-one(19)and 6β-hydroxystigmast-4-en-3-one(20),respectively.Among them,compounds 1 and 2 are new compounds,compounds 6,9,18 and 20 are isolated from Annonaceae plants for the first time and compounds 5,7,17 and 19 are isolated from the genus Miliusa for the first time.Human triple negative breast cancer cells(MDA-MB-231 and BT-549)were used to evaluate the antitumor activity of these isolates by MTT assay.Compounds 10 and 11 showed significant antiproliferation activity against the test cells with half inhibition concentration(IC50)of 0.98-4.85μmol·L-1.

关 键 词：云南野独活 番荔枝科 化学成分 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]