连翘中一个新的半日花烷型二萜类化合物  

A new labdane diterpene compound in Forsythia suspensa(Thunb.)Vahl

作　　者：陈晓兰 杨青青 陈宣钦[1] 李蓉涛[1] 张治军 CHEN Xiao-lan;YANG Qing-qing;CHEN Xuan-qin;LI Rong-tao;ZHANG Zhi-jun(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《药学学报》2025年第3期755-761,共7页Acta Pharmaceutica Sinica

基　　金：云南省基础研究计划(202101BE070001-045);云南省重大科技专项(202302AG050004)资助项目。

摘　　要：采用多种色谱分离技术(硅胶柱色谱、MCI柱色谱、Sephadex LH-20凝胶柱色谱等)对木樨科(Oleaceae)连翘属植物连翘(Forsythia suspensa)的果实进行化学成分研究。从其95%乙醇提取部分中分离得到14个萜类化合物,并通过MS、1D-和2D-NMR等波谱手段和量子化学计算方法进行了结构鉴定:半日花烷型二萜(1~6)、克罗烷型二萜(7)、降半日花烷型二萜(8)、降克罗烷型二萜(9)、齐墩果烷型三萜(10)、乌苏烷型三萜(11,12)、羽扇豆烷型三萜(13,14)。其中化合物1为新化合物,化合物3~9、11和12为首次从中药连翘中分离得到。评价了这些化合物对脂多糖(LPS)诱导小鼠巨噬细胞RAW 264.7产生一氧化氮(NO)的抑制作用,结果显示这些化合物均未表现出明显的抑制活性。The chemical constituents of the fruits of Forsythia suspensa(Thunb.)Vahl were investigated by using chromatographic techniques(silica gel,MCI,and sephadex LH-20 gel column chromatography,etc).Fourteen compounds were isolated from the 95%ethanol extract of F.suspensa,and their structures were identified by HR-ESI-MS,NMR,and calculated electronic circular dichroism(ECD)methods.The compounds included labdane diterpenes(1-6),clerodane diterpene(7),norlabdane diterpene(8),norclerodane diterpene(9),oleanane triterpenoid(10),ursane triterpenoids(11,12),lupane triterpenoids(13,14).Among them,compound 1 was a new compound and compounds 3-9,11,and 12 were obtained from this plant for the first time.The inhibitory effect of these compounds on lipopolysaccharide-induced nitric oxide production in mouse macrophage RAW 264.7 cells also was evaluated.Unfortunately,none of these compounds exhibited significant inhibitory activity.

关 键 词：连翘 半日花烷型二萜 巨噬细胞RAW 264.7 一氧化氮抑制生成活性 

分 类 号：R284[医药卫生—中药学]