UPLC-Q-TOF/MS^(E)分析鉴定肝豆扶木汤化学成分  

Identification of chemical components in Gandoufumu Decoction by UPLC-Q-TOF/MS^(E)

作　　者：黄蕾 王倪 董婷[2] 曹仕健[2] 蒋羽鸽 吴欢[3,4] 周安 HUANG Lei;WANG Ni;DONG Ting;CAO Shi-jian;JIANG Yu-ge;WU Huan;ZHOU An(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;Key Laboratory of Xin'An Medicine,Ministry of Education,Hefei230012,China;Functional Activity and Resource Utilization on Edible and Medicinal Fungi Joint Laboratory of Anhui Province,Lu'an 237399,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽中医药大学第一附属医院,安徽合肥230031 [3]新安医学教育部重点实验室,安徽合肥230012 [4]食药用菌功能活性与资源利用安徽省联合共建学科重点实验室,安徽六安237399

出　　处：《药学学报》2025年第3期771-783,共13页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(81872976);安徽省卫生与健康研究(AHWJ2022b062);安徽省红十字会中医药传承创新与发展研究项目(2022ZYYD06);安徽省临床医学研究转化专项(202204295107020043)。

摘　　要：通过超高效液相色谱串联四极杆飞行时间质谱技术(UPLC-Q-TOF/MS^(E))对肝豆扶木汤的化学成分进行分析鉴定;采用ZORBAX RRHD Eclipse Plus C_(18)色谱柱(2.1 mm×100 mm,1.8μm),以0.1%甲酸水-乙腈为流动相进行梯度洗脱,流速为0.2 m L·min^(-1),柱温35℃。质谱分析采用电喷雾电离源(ESI)的方式,在正、负离子模式下,通过UPLC-Q-TOF/MS^(E)分析鉴定肝豆扶木汤的化学成分。共鉴定出102种化合物,其中黄酮类成分26个,萜类成分22个,皂苷类成分19个,苯丙素类成分10个和其他类成分25个。本研究所建立的定性分析方法能快速、高效地对肝豆扶木汤的化学成分进行分析鉴定,为肝豆扶木汤的质量评价和临床应用提供了科学依据。The main chemical components of Gandoufumu Decoction(GDFMD)were analyzed and identified using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS^(E)).We utilized a ZORBAX RRHD Eclipse Plus C_(18)column(2.1 mm×100 mm,1.8μm)with a gradient elution of 0.1%formic acid in water and acetonitrile as mobile phase at a flow rate of 0.2 m L·min^(-1)and a column temperature of35℃.The mass spectrometry was analyzed using an electrospray ionization source(ESI)in positive and negative ion modes.The UPLC-Q-TOF/MS^(E) was used to detect and analyze the complex mass spectra and the chemical composition of the GDFMD.The results show that 102 compounds were identified in GDFMD,26 flavonoids,22terpenoids,19 saponins,10 phenylpropanoids,and 25 other components.The established qualitative method can rapidly and efficiently identify the chemical components of GDFMD,providing a scientific basis for the quality evaluation and clinical application of GDFMD.

关 键 词：肝豆扶木汤 UPLC-Q-TOF/MS^(E) 化学成分 定性分析 裂解规律 

分 类 号：R917[医药卫生—药物分析学]