九制多花黄精对雌性大鼠慢性不可预知应激模型的预防效果  

Effect of Processed Polygonatum cyrtonema in Preventing Depression Induced by Chronic Unpredictable Mild Stress in Female Rats

作　　者：邓欣雨 马春花 王梓萌 唐漫 李昕燃 喻录容 何先元[1,5] DENG Xinyu;MA Chunhua;WANG Zimeng;TANG Man;LI Xinran;YU Lurong;HE Xianyuan(College of Traditional Chinese Medicine(TCM),Chongqing Medical University,Chongqing 401331,China;College of Acupuncture and Massage,Chengdu University of TCM,Chengdu 610075,China;Chongqing Key Laboratory of Prevention and Treatment of Metabolic Diseases with TCM,Chongqing 400016,China;School of Chinese Materia Medica,Chongqing College of TCM,Chongqing 402760,China;School of Pharmacy,Xinjiang Second Medical College,Karamay 834000,China)

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]成都中医药大学针灸推拿学院,成都610075 [3]中医药防治代谢性疾病重庆市重点实验室,重庆400016 [4]重庆中医药学院中药学院,重庆402760 [5]新疆第二医学院药学院,新疆克拉玛依834000

出　　处：《中国实验方剂学杂志》2025年第7期117-124,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆科技委员会的社会民生项目(cstc2018jscx-msybX0288);重庆科学与健康联盟中医药技术创新与应用开发重点项目(2021ZY013872);重庆医科大学大学生科学研究与创新实验项目(SRIEP202181)。

摘　　要：目的:观察九制多花黄精对慢性不可预知应激模型(CUMS)造模进行中雌性大鼠的抑郁预防控制效果。方法:将40只SPF级SD雌性大鼠根据随机数字表法分为正常组,模型组,九制黄精低、中、高浓度预防组,每组8只。采用CUMS造模法,通过体质量、旷场实验、强迫游泳实验、神经递质[多巴胺(DA)、5-羟色胺(5-HT)、乙酰胆碱(ACh)]、血清性相关激素[促性腺激素释放激素(GnRH)、睾酮(T)、雌二醇(E_(2))]、血清炎症因子[肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)]、脑源性神经营养因子(BDNF)/酪氨酸激酶受体B(TRKB)/环磷腺苷效应元件结合蛋白(CREB)通路mRNA蛋白表达等各项指标评价九制多花黄精对预防雌性大鼠CUMS抑郁模型的影响。结果:从大鼠体质量、旷场实验、强迫游泳实验等开始造模前后行为的比较推测模型组大鼠抑郁已建立,通过对各处理组间行为学、神经递质、性相关激素、炎症因子和神经通路的比较,可以得出药物应用对预防雌性大鼠抑郁症进展均有不同程度的作用效果。采用SPSS 25软件进行误差和显著性统计分析,组间用t检验,与模型组比较,各处理组作用效果明显(P<0.05)。九制黄精显著提升雌性大鼠脑组织5-TH水平(P<0.01),显著降低DA、ACh水平(P<0.01),明显降低大鼠血清GnRH、T、E_(2)、TNF-α、IL-6水平(P<0.05),提高大鼠大脑BDNF、TRKB mRNA表达。结论:九制多花黄精对大鼠CUMS抑郁模型有非亢进性预防效果,为将九制多花黄精开发成功能性食品提供了理论依据。Objective:To observe the prevention and control effect of processed Polygonatum cyrtonema on depression induced by chronic unpredictable mild stress(CUMS)in female rats.Methods:Forty rats were assigned into control,model,and low-,medium-,and high-dose processed P.cyrtonema groups according to the random number table method,with 8 rats in each group.The rat model of depression was established with the CUMS method.The body mass,open field test,forced swimming test,Morris water maze test,levels of neurotransmitters[dopamine(DA),5-hydroxytryptamine(5-TH),and acetylcholine(ACh)],serum levels of sex hormones[gonadotropin-releasing hormon(e GnRH),testosterone(T),and estradiol(E_(2))]and inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-10],and mRNA and protein levels of factors in the brainderived neurotrophic factor(BDNF)/tyrosine kinase receptor B(TRKB)/cAMP-response element binding protein(CREB)pathway were employed to evaluate the effect of processed P.cyrtonema on the CUMS-induced depression in female rats.Results:The body mass,open field test results,and forced swimming test results showed that the rat model of depression was successfully established.The comparison of behaviors,neurotransmitters,sex hormones,inflammatory factors,and neural pathways among groups showed that processed P.cyrtonema had different effects of preventing the development of depression in female rats.SPSS 25 was used for statistical analysis of error and significance.T test was conducted between groups.Each treatment group showed significant therapeutic effect compared with the model group(P<0.05).Processed P.cyrtonema elevated the level of 5-TH(P<0.01)and lowered the levels of DA and ACh(P<0.01)in the brain tissue of female rats.In addition,it reduced the serum levels of GnRH,T,E_(2),TNF-α,and IL-6(P<0.05)and up-regulated the mRNA levels of BDNF and TRKB in the rat brain.Conclusion:Processed P.cyrtonema has a non-hyperactive preventive effect on CUMS-induced depression in rats,which provides a theoretical basi

关 键 词：抑郁症 预防 九制多花黄精 雌性大鼠 慢性不可预知应激模型(CUMS)抑郁模型 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R277.7