基于胃“炎-癌”转化探讨IL-13Rα1与COX-2蛋白表达的变化及意义  

Exploring the changes and significance of IL-13Rα1 and COX-2 protein expression based on gastric"inflammation-cancer"transition

作　　者：倪慕兰 陈启斌 李燕维 陈永东 王宇航 NI Mulan;CHEN Qibin;LI Yanwei;CHEN Yongdong;WANG Yuhang(Department of Pathology,Fuding Hospital,Fujian University of Traditional Chinese Medicine,Fuding,Fujian,355200,China;District 1 of Internal Medicine of Traditional Chinese Medicine,Fuding Hospital,Fujian University of Traditional Chinese Medicine)

机构地区：[1]福建中医药大学附属福鼎医院病理科,福建福鼎355200 [2]福建中医药大学附属福鼎医院中医内科一区

出　　处：《中国中西医结合消化杂志》2025年第1期52-57,62,共7页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：福建中医药大学校管科研课题临床专项(No:XB2022053)。

摘　　要：目的:检测胃“炎-癌”转化各关键阶段组织样本中幽门螺杆菌(Helicobacter pylori,HP)、白介素13受体α1(interleukin-13 receptorα1,IL-13Rα1)及环氧合酶-2(cyclooxygenase-2,COX-2)等蛋白的表达,探讨其在“炎-癌”转化中的作用及意义。方法:将研究对象分为6个组:20例正常胃黏膜(N组)、50例慢性非萎缩性胃炎(CNAG组)、50例慢性萎缩性胃炎伴肠上皮化生(CAG/IM组)、50例胃黏膜低级别异型增生(LGD组)、50例胃黏膜高级别异型增生(HGD组)和50例胃癌(GC组),采用免疫组织化学EnVision两步法检测活检组织中HP、IL-13Rα1及COX-2蛋白的表达。结果:HP在N组、CNAG组、CAG/IM组、LGD组、HGD组和GC组中阳性表达率分别为15%、38%、48%、46%、42%、36%,各组间比较差异无统计学意义(P>0.05);IL-13Rα1在各组中的阳性表达率呈逐渐上升趋势,分别为5%、18%、62%、78%、86%、92%,IL-13Rα1阳性表达与病变分级呈正相关(P<0.01)。IL-13Rα1在CAG/IM组、LGD组、HGD组、GC组高表达,与N组、CNAG组比较差异有统计学意义(P<0.01),而在CAG/IM组、LGD组、HGD组、GC组间差异无统计学意义(P>0.05);COX-2在各组中的阳性表达率分别为5%、4%、12%、24%、36%、84%,在GC组显著高表达,与N组、CNAG组、CAG/IM组、LGD组、HGD组比较差异有统计学意义(P<0.01)。经Spearman等级相关性分析,胃癌组织中COX-2的表达与IL-13Rα1的表达呈正相关(r=0.475,P<0.01)。结论:IL-13Rα1可能是慢性胃炎向胃上皮化生发生的早期关键分子,而COX-2可能在化生后进一步发生癌变中起作用,二者有助于胃“炎-癌”转化风险评估及病情监测。Objective To investigate the expression of Helicobacter pylori(HP),interleukin-13 receptorα1(IL-13Rα1),and cyclooxygenase-2(COX-2)proteins in tissue samples representing key stages of gastric inflammation and cancer transformation,and to explore their roles and significance in the"inflammation-cancer"transition.Methods We used immunohistochemical EnVision two-step method to detect and analyze the protein expression of HP,IL-13Rα1,and COX-2 in 270 biopsy tissue samples.These samples were categorized into six groups:normal gastric mucosa(N group,n=50),chronic non-atrophic gastritis(CNAG group,n=50),chronic atrophic gastritis with intestinal metaplasia(CAG/IM group,n=50),low-grade dysplasia of gastric mucosa(LGD group,n=50),high-grade dysplasia of gastric mucosa(HGD group,n=50),and gastric cancer(GC group,n=50).Results The positive expression rates of HP in the N,CNAG,CAG/IM,LGD,HGD,and GC groups were 15%,38%,48%,46%,42%,and 36%,respectively.There was no statistically significant difference in HP expression between the groups(P>0.05).The positive expression rates of IL-13Rα1 in each group showed a gradual increasing trend:5%,18%,62%,78%,86%,and 92%,respectively.IL-13Rα1 expression was positively correlated with lesion grading(P<0.01).IL-13Rα1 was highly expressed in the CAG/IM,LGD,HGD,and GC groups,showing significant differences compared to the N and CNAG groups(P<0.01),while there was no significant difference between the CAG/IM,LGD,HGD,and GC groups(P>0.05).The positive rates of COX-2 in each group were 5%,4%,12%,24%,36%,and 84%,respectively.COX-2 was significantly overexpressed in the GC group compared to the N,CNAG,CAG/IM,LGD,and HGD groups(P<0.01).Pearson correlation analysis revealed a positive correlation between IL-13Rα1 and COX-2 expression in gastric cancer tissue(r=0.475,P<0.01).Conclusion IL-13Rα1 may be an early key molecule in the occurrence of gastric epithelial metaplasia in chronic gastritis,while COX-2 may play a role in further carcinogenesis after metaplasia.Both contribute to the risk a

关 键 词：白介素13受体α1 环氧合酶-2 胃“炎-癌”转化 肠上皮化生 癌前病变 免疫组织化学 

分 类 号：R256.3[医药卫生—中医内科学]