基于石墨烯折射率传感技术检测膀胱癌标志物miRNA-21  

Detection of bladder cancer marker miRNA-21 based on graphene refractive index sensing technology

作　　者：许文瑞 高晓光 李金 王颖倩 张校亮 李晓春 XU Wenrui;GAO Xiaoguang;LI Jin;WANG Yingqian;ZHANG Xiaoliang;LI Xiaochun(College of Biomedical Engineering,Institute of Biomedical Precision Testing and Instrumentation,Taiyuan University of Technology,Jinzhong 030600,China)

机构地区：[1]太原理工大学生物医学工程学院,生物医学精准检测与仪器研究所,晋中030600

出　　处：《分析试验室》2025年第2期159-166,共8页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(62005189,22104108,61775157,21874098);山西省自然科学基金(20210302124001,201901D211071);山西省“四个一批”科技兴医创新计划-山西省医学重点科研项目(2022XM17)资助。

摘　　要：本研究基于石墨烯折射率传感技术,并结合金纳米颗粒(AuNPs)的局域表面等离子体共振效应,构建了膀胱癌标志物miRNA-21的检测方法,其折射率分辨率达到4.56×10^(-9)。在石墨烯表面固定探针链,并合成与探针链互补配对的核酸序列功能化的AuNPs。AuNPs的局域表面等离子体共振效应会扰动石墨烯表面的倏逝场,引起石墨烯表面折射率的明显变化,从而实现信号放大。通过检测DNA-AuNPs和目标物竞争性结合探针链时产生的信号变化,实现对miRNA-21的高灵敏度检测。在无需扩增的情况下,该传感技术对于膀胱癌标志物miRNA-21的检出限和线性范围分别为0.41 pmol/L和0.50~10 pmol/L。此外,该传感技术还表现出了优异的特异性,能够成功识别单碱基错配。利用该传感技术对尿液样本进行加标回收测定,加标回收率为107.1%~117.5%。该传感技术能够实时监测核酸动态反应过程,可为膀胱癌标志物miRNA-21的定量检测提供参考。In this study,a method was constructed for detecting the bladder cancer marker miRNA-21 based on graphene refractive index sensing technology combined with the local surface plasmon resonance effect of gold nanoparticles(AuNPs),and its refractive index resolution reached 4.56 x 10^(-9).The probe chains were immobilized on the graphene surface,and AuNPs functionalized with nucleic acid sequences complementary to the probe strands were synthesized.The localized surface plasmon resonance of AuNPs will disturb the evanescent field on the graphene surface,causing a significant change in the refractive index,thereby achieving signal amplification.Highly sensitive detection of miRNA-21 can be achieved by detecting the signal changes generated when DNA-AuNPs and the target competitively bind to the graphene surface probe strand.Without the amplification process,the detection limit and linear range of this sensing technology for the bladder cancer marker miRNA-21 are 0.41 pmol/L and 0.50-10 pmol/L,respectively.In addition,the sensing technology also exhibits excellent specificity and could successfully identify single-base mismatches.This sensing technology was used to perform spiked recovery measurements on urine samples,and the spiked recovery rates were 107.1%-117.5%.This sensing technology can monitor the dynamic reaction process of nucleic acids in real time,and provides a reference for the quantitative detection of the bladder cancer marker miRNA-21.

关 键 词：MIRNA 膀胱癌 石墨烯 折射率传感 表面等离子体共振 

分 类 号：O657.1[理学—分析化学] X832[理学—化学]