罗丹明-苯硼酸酰腙荧光探针的合成及对铜离子和汞离子的检测  

Synthesis of rhodamine-phenylboronic acylhydrazone fluorescent probe for detection of copper ions and mercury ions

作　　者：包晓英 邵雨昕 杨杨[1] 王曦烨[1] 李鹏 高超颖[1] BAO Xiaoying;SHAO Yuxin;YANG Yang;WANG Xiye;LI Peng;GAO Chaoying(Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis,Inner Mongolia Minzu University,Tongliao 028000,China;School of Sciences,Beihua University,Jilin 132013,China)

机构地区：[1]内蒙古自治区天然产物化学及功能分子合成重点实验室,内蒙古民族大学化学与材料学院,通辽028000 [2]北华大学理学院,吉林132013

出　　处：《分析试验室》2025年第2期199-204,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(22061033,82260844);内蒙古自然科学基金(2019MS02004);内蒙古直属高校基本科研业务费(GXKY22117)资助。

摘　　要：通过罗丹明酰肼与4-甲酰基苯硼酸的缩合反应制备了探针分子RbBA,并利用紫外-可见(UV-vis)吸收光谱和荧光光谱对其传感性能进行表征。研究了RbBA与Cu^(2+)和Hg^(2+)之间的相互作用过程,并探讨了其在荧光成像方面的应用。实验结果表明,在80%乙醇水溶液中,RbBA对Cu^(2+)和Hg^(2+)表现出高选择性吸收和荧光响应,且光谱强度与离子浓度呈良好的线性关系。循环实验和Job′s plot实验证明RbBA与Cu^(2+)/Hg^(2+)在配位过程中形成了1∶1的络合物。该探针具有较好的生物相容性,可用于活体内Hg^(2+)的识别。In this study,a rhodamine fluorescent probe(RbBA)was synthesized by condensation of rhodamine hydrazide with 4-formylphenylboronic acid,and its sensing performance was evaluated by ultraviolet‒visible(UV-vis)absorption spectroscopy and fluorescence spectroscopy.The interaction processes of RbBA with copper ions/mercury ions(Cu^(2+)/Hg^(2+))and the probe´s application in fluorescence imaging were investigated.The experimental results demonstrated that the probe RbBA exhibited high selectivity towards Cu^(2+)/Hg^(2+)ions and high fluorescence response in 80%ethanol solution.There was a good linear relationship between the spectral intensity and ion concentration.Cyclic tests and Job´s plot experiments confirmed the formation of 1∶1 complex in the coordination process of RbBA with Cu^(2+)/Hg^(2+).The probe demonstrates good biocompatibility and is suitable for in vivo recognition of Hg^(2+).

关 键 词：苯基硼酸 罗丹明酰腙 铜离子和汞离子检测 荧光成像 

分 类 号：O626[理学—有机化学] O641[理学—化学]