表面增强拉曼光谱法测定硒软胶囊中硒代蛋氨酸含量  

Determination of selenomethionine in selenium soft capsules by surface enhanced Raman spectroscopy

作　　者：刁全平 郭华 罗维巍 孙丽阳 吕琳琳 李铁纯 侯冬岩 DIAO Quanping;GUO Hua;LUO Weiwei;SUN Liyang;LV Linlin;LI Tiechun;HOU Dongyan(Liaoning Key Laboratory of Development and Utilization for Natural Products Active Molecules,School of Chemistry and Life Science,Anshan Normal University,Anshan 114007,China)

机构地区：[1]辽宁省天然产物活性分子开发及利用重点实验室,鞍山师范学院化学与生命科学学院,鞍山114007

出　　处：《分析试验室》2025年第2期273-277,共5页Chinese Journal of Analysis Laboratory

基　　金：辽宁省教育厅科学研究项目(SYSF202012)资助。

摘　　要：建立了表面增强拉曼光谱(SERS)检测硒软胶囊中硒代蛋氨酸(SeMet)含量的方法。以银纳米粒子溶胶(C-AgNPs)为SERS基底,1 moL/L NaCl为C-AgNPs的聚沉剂,以561 cm^(-1)和593 cm^(-1)处的峰为SeMet的SERS特征峰。在此条件下,561 cm^(-1)和593 cm^(-1)处的SERS峰面积和与SeMet的浓度在12.5~125 mg/L范围内呈良好的线性关系,相关系数R^(2)=0.9967,相对标准偏差(RSD)小于4.5%,平均回收率在90.0%~98.7%之间。该方法可用于硒软胶囊中SeMet的含量检测。A method was established for the determination of selenomethionine(SeMet)in selenium soft capsules by surface enhanced Raman spectroscopy(SERS)using colloidal silver nanoparticles(C-AgNPs)as the SERS substrate and 1 moL/L NaCl as the precipitator.The peaks at 561 cm^(-1)and 593 cm^(-1)were used as the characteristic peaks of the Raman spectrum of SeMet.Under these conditions,the total SERS peak area of 561 cm^(-1)and 593 cm^(-1)showed a good linear relationship with the concentration of SeMet in the range of 12.5-125 mg/L,with the correlation coefficient R^(2)=0.9967.The relative standard deviations(RSDs)were lower than 4.5%and the average recoveries were 90.0%-98.7%.This method can be used to detect content of SeMet in selenium soft capsules.

关 键 词：硒代蛋氨酸 表面增强拉曼光谱 银纳米粒子溶胶 

分 类 号：O657.37[理学—分析化学]