基于孟德尔随机化分析肠道菌群与肠套叠的因果关系  

Causal relationship between intestinal flora and intussusception based on Men-delian randomization analysis

作　　者：罗春 程丰 尚克磊 谭小光 王后红 徐梦雷 李彬 Luo Chun;Cheng Feng;Shang Kelei;Tan Xiaoguang;Wang Houhong;Xu Menglei;Li Bin(Department of Pediatric Surgery,Fuyang Women and Children's Hospital,Anhui,Fuyang 236000,China)

机构地区：[1]阜阳市妇女儿童医院小儿外科,安徽阜阳236000

出　　处：《发育医学电子杂志》2025年第2期109-113,共5页Journal of Developmental Medicine (Electronic Version)

基　　金：阜阳师范大学科学研究项目(2021HXYXZX10)。

摘　　要：目的通过孟德尔随机化(Mendelian randomization,MR)研究肠道菌群与肠套叠的关系。方法使用来自MiBioGen和FinnGen联盟的全基因组关联研究(Genome-wide Association Study,GWAS)汇总统计数据,进行双样本MR分析。通过逆方差加权法(inverse variance weighted,IVW)、MR-Egger回归法、加权中位数(weighted median estimator,WME)法、加权模型和MR-PRESSO回归法等分析肠道菌群与肠套叠之间的因果关系。结果共纳入211个菌群的3631个工具变量。IVW法共检测出5种肠道细菌与肠套叠有关,其中双歧杆菌对肠套叠的发生反向相关(OR=0.376,95%CI:0.157~0.899),瘤胃球菌(OR=1.461,95%CI:1.058~2.807)、阿德勒克罗伊茨菌(OR=1.893,95%CI:0.318~3.502)、蒜头藻菌(OR=1.715,95%CI:1.009~2.915)、梭状芽孢杆菌(OR=1.974,95%CI:1.046~4.451)与肠套叠的发生正向相关。MR-PRESSO回归分析与MR-Egger回归分析结果表明,不存在异质性及水平多效性。结论双歧杆菌可能降低肠套叠及术后复套的发生风险,瘤胃球菌、阿德勒克罗伊茨菌、蒜头藻菌、梭状芽孢杆菌可能增加肠套叠的发病风险。Objective To explore the relationship between intestinal flora and intussusception based on Mendelian randomization(MR).Method Using summary statistics from Genome-wide Association Studies(GWAS)conducted by the MiBioGen and FinnGen consortia,a two-sample MR analysis was performed.The causal relationship between intestinal flora and intussusception was analyzed using methods such as inverse variance weighted(IVW),MR-Egger regression,weighted median estimator(WME),weighted mode,and MRPRESSO regression.Result A total of 3631 instrumental variables from 211 microflora were included.A total of 5 types of intestinal bacteria were associated with intussusception detected by IVW method,among which Bifidobacterium had a reverse correlation with intussusception(OR=0.376,95%CI:0.157-0.899),Ruminococcus(OR=1.461,95%CI:1.058-2.807),Adlercreutzia(OR=1.893,95%CI:0.318-3.502),Allisonella(OR=1.715,95%CI:1.009-2.915),Clostridium(OR=1.974,95%CI:1.046-4.451)were positively correlated with the incidence of intussusception.The results of MR-PRESSO and MR-Egger showed that there was no heterogeneity and horizontal pleiotropy.Conclusion Bifidobacterium may reduce the risk of intussusception and postoperative recurrent intussusception.Ruminal cocci,Adlercreutzia,Allisonella,and Clostridium may increase the risk of intussusception.

关 键 词：肠套叠 肠道菌群 孟德尔随机化 因果关系 双歧杆菌 

分 类 号：R72[医药卫生—儿科]