苍耳子含药血清对IgE致肥大细胞脱颗粒、炎性因子释放及PLCγ、PKC表达的影响  

Effects of Cang’erzi(Xanthium xanthii)-Containing Serum on Mast Cells Degranulation Induced by IgE and Infalmmatory Factors,PLCγ、PKC Expressions

作　　者：周淑贞 邵深深 ZHOU Shuzhen;SHAO Shenshen(Department of Traditional Chinese Medicine,Ningbo Branch of Renji Hospital,Medicine Schiool of Shanghai Jiaotong University,Ningbo Hangzhou Bay Hospital,Ningbo,Zhejiang 315336;Department of Pharmacy,Wenzhou People’s Hospital,Wenzhou,Zhejiang 325000)

机构地区：[1]上海交通大学医学院附属仁济医院宁波医院,宁波市杭州湾医院中医科,浙江宁波315336 [2]温州市人民医院药剂科,浙江温州325000

出　　处：《中国中医药科技》2025年第2期211-215,共5页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省温州市基础医疗卫生科技项目(Y20180817)。

摘　　要：目的:基于磷脂酶C-γ/蛋白激酶C/钙离子(PLCγ/PKC/Ca^(2+))信号通路探究苍耳子含药血清对肥大细胞(Mast Cells,MCs)脱颗粒的调控作用。方法:常规培养RBL-2H3细胞并用IgE致敏,实验分为空白对照组、模型组、苍耳子含药血清组及富马酸酮替芬对照组,各组给予相应药物干预,免疫荧光染色测定RBL-2H3细胞培养基上清液钙离子内流情况。酶联免疫吸附试验(ELISA)检测炎症因子TNF-α、IL-6释放量和β氨基己糖苷酶(β-Hex)含量变化。Western blot检测磷酸化的T-细胞激活连接蛋白(p-LAT)、磷酸化的磷酯酶Cα1(p-PLCα1)、p-PKC蛋白表达情况。结果:与空白对照组比较,模型组钙离子内流、TNF-α和IL-6释放量、细胞脱颗粒能力、PKCα的荧光表达强度以及p-LAT、p-PLCα1和p-PKC的蛋白表达水平均显著升高(P<0.01)。相比于模型组,苍耳子含药血清组及富马酸酮替芬组钙离子内流、TNF-α和IL-6释放量、细胞脱颗粒能力、PKCα的荧光表达强度显著降低(P<0.01);p-LAT、p-PLCα1和p-PKC的蛋白表达水平均显著降低(P<0.05或P<0.01)。结论:苍耳子含药血清可以通过PLCγ/PKC/Ca^(2+)信号通路调控MCs脱颗粒。Objective:To investigate the effects of Xanthium xanthii-containing serum on degranulation of Mast Cells(MCs)by regulating PLCγ/PKC expressions.Methods:RBL-2H3 cells were routinely cultured and sensitized with IgE,experiment was divided into control group,model group,Xanthium xanthii-containing serum group and Ketotifen fumarate group(positive group)to receive corresponding drug intervention.Determination of calcium influx in the supernatant of RBL-2H3 cell culture medium by immunofluorescence staining.ELISA to detect changes in the levels of the inflammatory factors TNF-α,IL-6 release andβ-aminohexosidase(β-Hex).Western blot was used to detect the expressions of phosphorylated T-cell-activated connexin(p-LAT),phosphorylated phosphodiesterase Cα1(p-PLCα1),and p-PKC proteins.Results:Compared with the control group,calcium influx,contents of TNF-αand IL-6,cell degranulation ability,fluorescence expression intensity of PKCαand protein expression levels of p-LAT,p-PLCα1 and p-PKC in model group were significantly increased(P<0.01).Compared with model group,calcium influx,TNF-αand IL-6 contents,cell degranulation ability and fluorescence expression intensity of PKCαin Xanthium xanthii-containing serum group and Ketotifen fumarate group were significantly decreased(P<0.01).The protein expression levels of p-LAT,p-PLCα1 and p-PKC were significantly decreased(P<0.05 or P<0.01).Conclusion:The Xanthium xanthii-containing serum can regulate MCs degranulation through PLCγ/PKC/Ca^(2+)signaling pathway.

关 键 词：苍耳子含药血清 肥大细胞脱颗粒 PLCγ/PKC/Ca^(2+)信号通路 TNF-α IL-6 体外实验 

分 类 号：R285.5[医药卫生—中药学]