火麻仁对SW620结肠癌细胞ATAD2表达的抑制作用及化学成分研究  

Study on the Inhibition of Huomaren(Cannabis Fructus)on ATAD2 Expression in SW620 Colon Cancer Cells and Its Components

作　　者：辛杨 杨清竹[2] 王海军 王伟明[1] 赵明 XIN Yang;YANG Qingzhu;WANG Haijun;WANG Weiming;ZHAO Ming(Heilongjiang Academy of Chinese Medicine Sciences,Harbin,Heilongjiang 150036;Qiqihar University,Qiqihar,Heilongjiang 161006;School of General Medicine and Continuing Education,Qiqihar Medical College,Qiqihar,Heilongjiang 161006;Technology Innovation Center of Industrial Hemp,State Administration for Market Regulation,Qiqihar,Heilongjiang 161006)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036 [2]齐齐哈尔大学,黑龙江齐齐哈尔161006 [3]齐齐哈尔医学院全科医学与继续教育学院,黑龙江齐齐哈尔161006 [4]国家市场监督管理总局工业大麻技术创新中心,黑龙江齐齐哈尔161006

出　　处：《中国中医药科技》2025年第2期222-226,共5页Chinese Journal of Traditional Medical Science and Technology

基　　金：国家自然科学基金项目(82174060);黑龙江省省属高等学校基本科研业务费科研项目(145209505);黑龙江省博士后资助项目(LBH-Z19099)。

摘　　要：目的:探究火麻仁对SW620结肠癌细胞的抑制作用及其化学成分。方法:以RT-qPCR技术为检测手段,考察火麻仁乙醇提取液对结肠癌SW620细胞三磷酸腺苷家族蛋白2(ATPase family AAA domain-containing protein 2,ATAD2)基因表达水平的影响;采用超高效液相色谱/四级杆-静电场轨道阱质谱,分别对液相色谱流动相种类、柱温、梯度洗脱条件,质谱分辨率、喷雾电压等条件进行优化,对火麻仁乙醇提取液中的化学成分进行定性检测。结果:火麻仁乙醇提取液可抑制结肠癌SW620细胞ATAD2的基因表达;在火麻仁提取液中共定性检测到22个化学成分。结论:火麻仁乙醇提取液具有潜在的抗结肠癌活性,其潜在活性成分包括脂肪酸类、黄酮类及生物碱类。Objective:To explore the inhibitory effect of Cannabis Fructus(CF)on colon cancer SW620 cells as well as its components.Methods:The effect of CF on the ATAD2 gene expression in colon cancer SW620 cells was investigated by real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-qPCR).Ultra-high performance liquid chromatography/quadrupole-exactive orbitrap mass spectrometry(UHPLC/Q-Exactive Orbitrap MS)was used to detect the components in CF after optimizing the detection conditions including liquid chromatography mobile phase,column temperature,gradient elution conditions,mass spectrometry resolution,spray voltage,etc.Results:The extraction of CF could inhibit the gene expression of ATAD2 in colon cancer SW620 cells.There were total 22 components being qualified in CF.Conclusion:The CF extract possessed potential anti-colon cancer activity,and its active components include fatty acids,flavonoids and alkaloids.

关 键 词：火麻仁 ATAD2 化学成分 超高效液相色谱/四级杆-静电场轨道阱质谱 

分 类 号：R28[医药卫生—中药学]