野火病发病程度对叶面细菌群落多样性及基因功能的影响  

Influence of Wildfire Disease on the Diversity and Functions of Plant Phyllosphere Microbial Communities

作　　者：米其利 刘文仪 杨文武 李浩斌 许力 王翔飞 张建铎 尹华群[4] 高茜 MI Qili;LIU Wenyi;YANG Wenwu;LI Haobin;XU Li;WANG Xiangfei;ZHANG Jianduo;YIN Huaqun;GAO Qian(Technology Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,Yunnan,China;College of Environment and Ecology,Hunan Agricultural University,Changsha 410128,Hunan,China;Yuxi Cigarette Factory,Hongta Group,Yuxi 653100,Yunnan,China;School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,Hunan,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,中国云南昆明650231 [2]湖南农业大学环境与生态学院,中国湖南长沙410128 [3]红塔集团玉溪卷烟厂,中国云南玉溪653100 [4]中南大学资源加工与生物工程学院,中国湖南长沙410083

出　　处：《生命科学研究》2025年第1期77-85,共9页Life Science Research

基　　金：云南省烟草化学重点实验室开放课题(2021539200340244)。

摘　　要：为探究野火病发病程度对作物叶面细菌群落多样性和基因功能的影响,利用16S rRNA基因高通量测序技术,解析了野火病不同发病程度之间植株叶面细菌群落的组成和多样性差异,并通过PICRUSt工具预测了叶面细菌群落的基因功能。分析结果表明,随着野火病病情加重,叶面细菌群落的物种丰富度和多样性显著降低(P<0.01),叶面细菌群落的组成发生了显著改变(P<0.05);相应地,水培液中pH上升、总磷含量降低、速效钾含量先增加再降低。此外,植物叶面细菌群落的免疫系统疾病、信号分子和相互作用、外源性生物降解与代谢的相关性随着疾病严重加剧而减弱。综上所述,感野火病植株叶面细菌群落的结构和功能与病害程度密切相关,降低水培液中pH、提高总磷和速效钾含量可缓解野火病发病程度。To explore the impact of wildfire disease on phyllosphere microbial community diversity and function,16S rRNA gene high-throughput sequencing technology was used to analyze the differences in the composition and diversity of phyllosphere prokaryotic microbial communities among plants with varying degrees of wildfire disease.Additionally,PICRUSt software was employed to predict the gene function of phyllosphere bacterial communities.The results revealed that,with the worsening of wildfire disease,both species richness and diversity of the leaf surface bacterial communities decreased significantly(P<0.01),and the distribution and composition of the leaf surface bacterial communities changed significantly(P<0.05).Correspondingly,pH in the culture medium increased,total phosphorus content decreased,and available potassium content first increased and then decreased.Furthermore,the correlations of the plant phyllosphere prokaryotic microbial community with immune system diseases,signaling molecules and interaction,exogenous biodegradation,and metabolism weakened with increasing disease severity.In conclusion,changes in the structure and function of leaf surface bacterial communities are closely related to the severity of wildfire disease in affected plants,and the disease can be relieved by decreasing pH and increasing total phosphorus and available potassium contents in the hydroponic solution.

关 键 词：野火病 病害程度 叶面微生物 细菌群落结构 功能预测 

分 类 号：Q948.12[生物学—植物学] S432.42[农业科学—植物病理学]