板栗上虫瘿和成瘿枝的代谢组与转录组联合分析  

Combined Metabolome and Transcriptome Analysis of Galls and Gall-bearing Branches of Castanea mollissima

作　　者：陈婷婷 惠羽 朱道弘[2] 曾杨[2] 杨筱慧[1] CHEN Tingting;HUI Yu;ZHU Daohong;ZENG Yang;YANG Xiaohui(College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China;College of Life and Environmental Sciences,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081 [2]中南林业科技大学生命与环境科学学院,中国湖南长沙410004

出　　处：《生命科学研究》2025年第1期86-94,共9页Life Science Research

基　　金：国家重点研发计划项目(2018YFE0127100)。

摘　　要：虫瘿是由昆虫取食或产卵刺激引起植物组织细胞异常增殖和分化,从而形成的突起或瘤状结构。栗瘿蜂(Dryocosmus kuriphilus)虫瘿为其幼虫提供食物来源并保护其生长。营养假说认为,虫瘿为致瘿昆虫提供了丰富的营养物质,因此其虫瘿组织中的营养物质含量应高于成瘿部位,而次生代谢产物的含量则相反。前期研究发现,栗瘿蜂虫瘿的糖类等营养物质高于其成瘿部位,而单宁等代谢物质的含量则相反,与营养假说一致。有研究分别利用代谢组和转录组分析发现,虫瘿的可溶性糖等营养物质含量高于成瘿部位,黄酮等次生代谢产物的含量则相反,同时与光合作用相关的基因表达下调。但是,目前尚无转录组和代谢组联合分析瘿蜂虫瘿与其成瘿部位差异的报道。本文首次联合代谢组和转录组,基于通路富集分析,更加全面地比较了板栗(Castanea mollissima)上栗瘿蜂虫瘿与成瘿部位之间的差异代谢物和差异表达基因。结果显示,栗瘿蜂虫瘿的氨基酸和多肽等含量上调,可能与其生长发育有关;而黄酮含量下调,可能是栗瘿蜂为了生存启动的防御机制,这二者均与营养假说相符。此外,通路富集分析显示,与光合作用相关的大部分基因在虫瘿中表达下调,抑制了寄主植物的光合作用。Galls are abnormal proliferation and differentiation of plant tissue cells caused by insect feeding or oviposition stimuli,forming protrusions or tumor-like structures.The gall wasps(Dryocosmus kuriphilus)provide food and protection for their larvae within the galls.According to the nutrition hypothesis,galls provide rich nutrients to the gall-inducing insects,and as such,the nutrient content in galled tissues should be higher than that in non-galled tissues,while the content of secondary metabolites should be inversely related.Previous studies have found that the content of nutrients like sugars in the galls induced by D.kuriphilus are higher than in non-galled tissues,while the content of metabolites like tannins is conversely lower,which is consistent with the nutrition hypothesis.Metabolomic and transcriptomic studies have separately revealed that the content of nutrients such as soluble sugars is elevated in galls compared with non-galled tissues,whereas the content of secondary metabolites like flavonoids shows an opposite trend,accompanied by downregulation of photosynthesis-related gene expression.However,there are currently no reports on combined transcriptomic and metabolomic analysis of the differences between the galled and nongalled tissues.This is the first time to integrate metabolomics and transcriptomics to comprehensively compare differential metabolites and differentially expressed genes between galled and non-galled tissues of the host plant Castanea mollissima by Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.The results indicated that the levels of amino acids and peptides in the galls were upregulated,possibly related to the growth and development of D.kuriphilus,while the flavonoid content was downregulated,potentially reflecting a defensive mechanism initiated by the insect for survival.The results align with the nutrition hypothesis.Furthermore,KEGG pathway enrichment analysis showed that the expressions of most photosynthesis-related genes were down-regulated in galls,inh

关 键 词：栗瘿蜂 虫瘿 成瘿枝 代谢组 转录组 

分 类 号：Q948.12[生物学—植物学] S433[农业科学—农业昆虫与害虫防治]