白星花金龟保幼激素与蜕皮激素处理条件下内参基因筛选  

Reference Gene Selection Under Conditions of Juvenile Hormone and Ecdysone Treatment in Protaetia brevitarsis

作　　者：范新源 佟一杰[2] 徐小博 赵政宇 陈新 高梦洁 付恩泽 周歧海[1] 白明[2] FAN Xinyuan;TONG Yijie;XU Xiaobo;ZHAO Zhengyu;CHEN Xin;GAO Mengjie;FU Enze;ZHOU Qihai;BAI Ming(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guangxi Normal University,Guilin,Guangxi Zhuang Autonomous Region 541001,China;Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;College of Forestry and Protoculture,Ningxia University,Yinchuan 750021,China;College of Life Science,Cangzhou Normal University,Cangzhou,Hebei Province 061000,China)

机构地区：[1]广西师范大学,珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541001 [2]中国科学院动物研究所,动物生物多样性保护与有害生物综合治理重点实验室,北京100101 [3]宁夏大学林业与草业学院,银川750021 [4]沧州师范学院生命科学学院,河北沧州061000

出　　处：《四川动物》2025年第2期203-212,共10页Sichuan Journal of Zoology

基　　金：国家重点研发计划资助项目(2022YFC2601200);国家自然科学基金青年科学基金项目(32200354);中国博士后科学基金第71批面上资助项目(2022M713134)。

摘　　要：白星花金龟Protaetia brevitarsis幼虫是重要的农林害虫和经济昆虫。采用保幼激素(JH)和蜕皮激素(20E)处理白星花金龟幼虫,并通过GeNorm、NormFinder、ΔCt法和BestKeeper等方法评估了8个候选内参基因(Rps3、EF-1α、27A、AK、Act、GAPDH、Rps18、Tub)的表达稳定性。根据RefFinder的综合排名,Rps3、Rps18和EF-1α分别在JH、发育阶段和20E处理下表现出较高的稳定性,适合作为白星花金龟的内参基因;JH和20E水平的变化会影响内参基因的表达稳定性。研究结果为白星花金龟基因表达研究提供了可靠的内参基因参考,为农业生产及废物资源化利用提供了理论依据;另一方面,筛选的内参基因可支持JH类似物相关实验的数据标准化,为成虫防控提供帮助。The larva of white spotted flower chafer(Protaetia brevitarsis)holds significant importance both as an agricultural and forestry pest and as an insect with high economic value.In this study,the larvae were subjected to treatments with juvenile hormone(JH)and 20-hydroxyecdysone(20E),and the expression stability of 8 candidate reference genes(Rps3,EF-1α,27A,AK,Act,GAPDH,Rps18,and Tub)was evaluated using GeNorm,NormFinder,ΔCt,and BestKeeper.The results showed that,according to the comprehensive ranking index provided by RefFinder,Rps3,Rps18,and EF-1αdemonstrated remarkable stability under the treatment of JH,developmental stage and 20E,respectively,thereby qualifying them as reference genes for this species.Furthermore,this study also uncovered that alterations in the concentrations of JH and 20E could exert an influence on the expression stability of these reference genes.Consequently,the selection of appropriate reference genes under conditions involving hormone treatments is crucial for improving the accuracy of gene expression analysis.This study provides a set of reliable reference genes for conducting gene expression studies in P.brevitarsis and may lay a foundation for agricultural production and waste resource utilization.Additionally,the identified reference genes serve as valuable tools for data normalization in experiments involving JH analogs,thereby facilitating the control of adult pests.

关 键 词：白星花金龟 内参基因 QRT-PCR 保幼激素 蜕皮激素 

分 类 号：Q966[生物学—昆虫学]