黑龙江省天然黄檗种群遗传多样性SSR分析  

SSR Analysis of Genetic Diversity of Natural Phellodendron amurense Populations in Heilongjiang Province

作　　者：袁显磊 王雨童 周志军 刘忠玲 张建瑛 YUAN Xianlei;WANG Yutong;ZHOU Zhijun;LIU Zhongling;ZHANG Jianying(Heilongjiang Academy of Forestry,Heilongjiang Harbin 150081)

机构地区：[1]黑龙江省林业科学研究所,黑龙江哈尔滨150081

出　　处：《林业科技》2025年第2期14-18,共5页Forestry Science & Technology

基　　金：黑龙江省省属科研院所科研业务费项目(YB2022-03)。

摘　　要：为明确黑龙江省黄檗天然种群的遗传分化机制,揭示不同地区黄檗天然种群的遗传关系,本研究利用SSR分子标记对325份来自黑龙江省的黄檗群体进行遗传多样性和遗传结构分析,结果表明,黄檗期望杂合度(H_e)为0.481,观测杂合度(H_o)为0.422,信息指数(I)为0.965,说明黄檗遗传多样性较高,遗传分化系数为F_(st)=0.067,说明遗传分化水平也处于较高状态,AMOVA分析显示,黄檗遗传变异中4%发生在种群间,83%发生在个体间,说明种间变异小;对黄檗14个种群遗传结构分析,遗传分化系数p=0.031<0.05,说明黄檗分布在不同种群之间存在显著的遗传分化。In order to clarify the genetic differentiation mechanism of natural Phellodendron amurense populations and to reveal the genetic relationship of natural Phellodendron amurense populations in different regions,this study analyzed the genetic diversity and genetic structure of 325 Phellodendron amurense populations from Heilongjiang Province using SSR molecular markers.The results showed that the expected heterozygosity(He)of Phellodendron amurense was 0.481,the observed heterozygosity(Ho)was 0.422,and the information index(I)was 0.965,indicating that the genetic diversity of Phellodendron amurense was high.0.965,indicating that Phellodendron amurense has high genetic diversity,and the coefficient of genetic differentiation is Fst=0.067,indicating that the level of genetic differentiation is also in a high state.AMOVA analysis showed that 4%of Phellodendron amurense genetic variations occurred between populations,and 83%of the genetic variation occurred between individuals,indicating that interspecific variations were small;the analysis of 14 Phellodendron amurense populations of genetic structure,and the coefficient of genetic differentiation,P=0.031<0.05,indicated that Phellodendron amurense was distributed in different populations.This indicates that Phellodendron amurense is distributed among different populations with significant genetic differentiation.

关 键 词：黄檗 遗传多样性 SSR分析 

分 类 号：Q792.31[生物学—分子生物学]