Sustaining ethanol production from lime pretreated water hyacinth biomass using mono and co-cultures of isolated fungal strains with Pichia stipitis  

作　　者：Chinnathambi Pothiraj Ramasubramanian Arumugam Muthukrishnan Gobinath 

机构地区：[1]PG and Research Department of Botany,Alagappa Government Arts College(Alagappa University),Karaikudi,Tamilnadu 630003,India [2]Department of Microbiology,VHNSN College,Virudhunagar,Tamilnadu 626001,India

出　　处：《Bioresources and Bioprocessing》2014年第1期1-10,共10页生物资源与生物加工(英文)

摘　　要：Background:The high rate of propagation and easy availability of water hyacinth has made it a renewable carbon source for biofuel production.The present study was undertaken to screen the feasibility of using water hyacinth's hemicelluloses as a substrate for alcohol production by microbial fermentation using mono and co-cultures of Trichoderma reesei and Fusarium oxysporum with Pichia stipitis.Results:In separate hydrolysis and fermentation(SHF),the alkali pretreated water hyacinth biomass was saccharified by crude fungal enzymes of T.reesei,F.oxysporum and then fermented by P.stipitis.In simultaneous saccharification and fermentation(SSF),the saccharification and fermentation was carried out simultaneously at optimized conditions using mono and co-cultures of selected fungal strains.Finally,the ethanol production kinetics were analyzed by appropriate methods.The higher crystalline index(66.7%)and the Fourier transform infrared(FTIR)spectra showed that the lime pretreatment possibly increased the availability of cellulose and hemicelluloses for enzymatic conversion.In SSF,the co-culture fermentation using T.reesei and P.stipitis was found to be promising with a higher yield of ethanol(0.411 g g^(−1))at 60 h.The additional yield comparable with the monocultures was due to the xylanolytic activity of P.stipitis which ferments pentose sugars into ethanol.In SHF,the pretreatment followed by crude enzymatic hydrolysis and fermentation resulted in a significantly lesser yield of ethanol(0.344 g g^(−1))at 96 h.Conclusions:It is evident from the study that the higher ethanol production was attained in a shorter period in the co-culture system containing T.reesei and the xylose fermenting yeast P.stipitis.SSF of pretreated water hyacinth biomass(WHB)with P.stipitis instead of traditional yeast is found to be an effective biofuel production process.

关 键 词：Water hyacinth Hemicelluloses XYLOSE T.reesei F.oxysporum P.stipitis SSF 

分 类 号：F42[经济管理—产业经济]