Efficient production of l-menthol in a two-phase system with SDS using an immobilized Bacillus subtilis esterase  

作　　者：Jiang Pan Ngoc-Duy Dang Gao-Wei Zheng Bo Cheng Qin Ye Jian-He Xu 

机构地区：[1]Laboratory of Biocatalysis and Synthetic Biotechnology,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Road,Shanghai 200237,China [2]Sunflower Pharmaceutical Group Company Limited,85 Sihuan Road,Chongqing 408000,China

出　　处：《Bioresources and Bioprocessing》2014年第1期108-113,共6页生物资源与生物加工(英文)

基　　金：financial supports by the Ministry of Science and Technology,P.R.China(Nos.2011AA02A210 and 2011CB710800);the Open Fund of State Key Laboratory of Bioreactor Engineering(2060204),are gratefully acknowledged.

摘　　要：Background:levo-Menthol is an important flavoring chemical,which can be prepared by enantioselective enzymatic hydrolysis of dl-menthyl esters.A recombinant esterase(BsE)cloned from Bacillus subtilis 0554 shows excellent enantioselectivity to dl-menthyl acetate and has been immobilized using cross-linked enzyme aggregates.Though BsE has relatively high substrate tolerance,the conversion of dl-menthyl acetate decreased sharply with the increase of substrate loading from 1 to 3 M in mono-aqueous system,which might be due to the severe inhibition of enzyme activity at extremely high load of substrate or product.In this work,enzymatic hydrolysis of dl-menthyl acetate with an extremely high load using the immobilized CLEA-BsE was investigated in an organic-aqueous biphasic system containing surfactant to establish a promising bioprocess for large-scale production of l-menthol.Results:An efficient biphasic reaction system of pentanol-water containing sodium dodecyl sulfate(SDS)was developed for improving enantioselective hydrolysis of dl-menthyl acetate to produce l-menthol by immobilized BsE.Under the optimized reaction conditions,l-menthol was produced in>97%enantiomeric excess(ee)at a substrate load of up to 3.0 M with>40%conversion.Conclusions:All the positive features demonstrate the potential applicability of the bioprocess for the large-scale production of l-menthol.

关 键 词：L-MENTHOL Enantioselective hydrolysis Organic-aqueous biphasic system dl-Menthyl acetate Immobilized Bacillus subtilis esterase 

分 类 号：O62[理学—有机化学]