基于羟基氧化钴氧化酶特性比色法检测乙酰胆碱酯酶  

Colorimetric detection of acetylcholinesterase activity based on the oxidase-like CoOOH nanosheets

作　　者：赵灵芝[1] 赵柳 刘春叶[1] 张小清[1] 王龙杰 杨喆 ZHAO Ling-zhi;ZHAO Liu;LIU Chun-ye;ZHANG Xiao-qing;WANG Long-jie;YANG Zhe(Department of pharmacy,Xi'an Medical College,Xi'an 710021,China;Shaanxi Agricultural Inspection and Testing Center,Xi'an,710003,China)

机构地区：[1]西安医学院药学院,陕西西安710021 [2]陕西省农业检验检测中心,陕西西安710003

出　　处：《化学研究与应用》2025年第3期585-591,共7页Chemical Research and Application

基　　金：2023年陕西省大学生创新项目(S202311840059)资助;陕西省科技厅项目(2022JM-094)资助;陕西省科技厅社发项目(2024SFYBXM-661)资助;咸阳市科技局社发项目(L2023-ZDYF-SF-023)资助;西安医学院杰出人才青年计划配套项目(2021PT08)资助;2022年科研能力提升计划项目(2022NLTS072)资助;西安医学院2024年度大学生开发实验科研基金(2024DXS37,2024DXS38)资助。

摘　　要：本研究基于羟基氧化钴CoOOH和四甲基联苯胺(TMB)显色反应发展了检测乙酰胆碱酯酶的比色法。在最佳条件下,CoOOH-TMB显色体系的吸光度与乙酰胆碱酯酶活力在0.5~7.0 U·L^(-1)范围内呈线性关系,检出限可达0.15 U·L^(-1)。将CoOOH-TMB显色体系结合纸芯片构建的纸基比色体系RGB值与乙酰胆碱酯酶活力在3~20 U·L^(-1)范围内呈线性关系,检出限可达0.5 U·L^(-1)。所构建的比色法可用于人血清中乙酰胆碱酯酶的检测,在临床分析和即时检测中显示出巨大应用潜力。In this study,a colorimetric method for the detection of acetylcholinesterase(AChE)was developed based on the chromogenic reaction of CoOOH nanosheets and tetramethylbenzidine(TMB).Under the optimal conditions,the absorbance of CoOOHTMB system was linearly related to AChE activity in the range of 0.5~7.0 U·L^(-1),and the detection limit was 0.15 U·L^(-1).The paper-based colorimetric system,constructed from CoOOH-TMB system,displayed a good linear relationship between RGB value and AChE activity in the range of 3~20 U·L^(-1),and a detection limit of 0.5 U·L^(-1).The colorimetric method can be used for the determination of AChE in human serum,and has great application potential in clinical analysis and real-time detection.

关 键 词：羟基氧化钴纳米片层(CoOOH) 3 3' 5 5'-四甲基联苯胺(TMB) 生物硫醇 乙酰胆碱酯酶 纸基比色法 

分 类 号：O657.3[理学—分析化学]